›› 2017, Vol. 37 ›› Issue (9): 796-799.

• 基础研究 • 上一篇    下一篇

辣椒素对牙龈卟啉单胞菌生长及实验性牙周炎大鼠炎性因子分泌的影响

郑一远1,周益2,王慧明2   

  1. 1. 温州医科大学仁济学院
    2. 浙江大学附属口腔医院
  • 收稿日期:2017-03-06 修回日期:2017-04-24 出版日期:2017-09-28 发布日期:2017-09-22
  • 通讯作者: 王慧明 E-mail:huimingwang1960@163.com
  • 基金资助:
    AngII/ARB通过AngIIR/cAMP/PKA途径影响骨质疏松进程研究

Effects of capsaicin on porphyromonas gingivalis growth and inflammatory cytokine secretion in experimental rat periodontitis

  • Received:2017-03-06 Revised:2017-04-24 Online:2017-09-28 Published:2017-09-22
  • Supported by:
    AngII/ARB affect osteoporosis via AngIIR/cAMP/PKA pathway

摘要: 目的 研究辣椒素对牙龈卟啉单胞菌(porphyromonas gingivalis,pg)生长影响及对实验性牙周炎大鼠炎性因子分泌的影响。方法 选用pg标准株ATCC33277,采用对倍稀释法测定辣椒素对pg的最低抑菌浓度(minimum inhibitory concentration,MIC)和最低杀菌浓度(minimum bactericidal concentration,MBC)。采用菌落计数法,通过时间—杀菌曲线的变化,分析辣椒素对细菌生长速度的影响。将无菌的28号结扎线放置在大鼠右侧第一磨牙颈部牙槽骨周围,以制备实验性牙周病模型;采用ELISA法分析16 mg/L (MIC)辣椒素处理后实验动物牙周组织中TNFα、IL1β、IL4、IL6、IL10和IL12等细胞因子的表达变化。结果 采用SPSS 19.0软件包进行数据分析。辣椒素对pg的MIC和MBC分别为16 mg/L和64 mg/L;辣椒素能有效抑制pg的生长,较高浓度时对其还具有杀菌作用。辣椒素对pg的抑菌和杀菌能力呈现时间依赖性。实验性牙周炎动物模型提示,除IL4外,辣椒素明显抑制牙周组织中TNFα、IL1β、IL6和IL12等炎症因子的表达,但提高IL10等细胞因子的表达水平。结论 辣椒素对牙龈卟啉单胞菌的生长有明显的抑制作用。同时通过选择性改变牙周组织中TNFα、IL1β、IL6、IL10和IL12等的表达水平,辣椒素可缓解牙周炎的免疫炎症反应程度。

关键词: 牙龈卟啉单胞菌, 辣椒素, 生长, 实验性牙周炎

Abstract: Objective To investigate the effect of Cap on the pg growth and inflammatory cytokine secretion in experimental rat periodontitis. Methods Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Cap against pg (ATCC33277) were determined by microdilution technique. The effect of Cap on the growth rate of pg was assessed by timekill curve. By placing the sterile 28gauge wire ligature to the cervical portion of the right first molar, the experimental animal periodontitis was set up. The levels of TNFα, IL1β, IL4, IL6, IL10, IL12 in the periodontal tissues were analyzed by ELISA. Results Statistical analysis was performed using SPSS19.0 software. It was found that Cap showed the inhibitory effects on pg growth with MIC at 16mg/L and 64 mg/L, respectively. In low concentration, Cap inhibited pg growth, but in high concentration, Cap could directly kill bacteria. The inhibitory or killing effect of Cap was also in timedependent manners. In experimental periodontitis, except IL4, TNFα, IL1β, IL6, IL12 were significantly inhibited by Cap, but the expression of IL10 increased. Conclusion Cap can inhibit the growth of pg. By selectively changing the expression of TNFα, IL1β, IL6, IL10, IL12, Cap is able to relieve the immuneinflammatory reaction in the periodontitis.

Key words: porphyromonas gingivalis, capsaicin, growth, experimental periodontitis

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