牙周膜干细胞与根尖乳头干细胞成骨及成牙本质能力比较的实验研究

›› 2018, Vol. 38 ›› Issue (1): 5-9.

牙周膜干细胞与根尖乳头干细胞成骨及成牙本质能力比较的实验研究

马玉,段子文,李淑慧,吴佩玲

新疆医科大学第二附属医院

收稿日期:2017-07-17 修回日期:2017-08-11 出版日期:2018-01-28 发布日期:2018-01-31
通讯作者: 吴佩玲 E-mail:wplkq@sina.com
基金资助:
国家自然科学基金项目（81460103）;新疆医科大学研究生创新创业项目（CXCY2017044）

Comparison of osteogenesis and dentinogenesis between periodontal ligament stem cells and apical papilla stem cells

Received:2017-07-17 Revised:2017-08-11 Online:2018-01-28 Published:2018-01-31

摘要/Abstract

摘要： ［摘要］目的体外培养人根尖乳头干细胞（Stem cells from apical papilla，SCAP）与牙周膜干细胞（periodontal ligament stem cells, PDLSCs），比较两者成骨及成牙本质能力，为干细胞应用于牙根再生研究提供理论依据。方法采用酶消化结合组织块化法获得SCAP及PDLSCs，通过流式细胞仪检测间充质干细胞表面标记物CD90、CD146的表达，采用CCK-8法测定SCAP及PDLSCs生长曲线；通过茜素红染色、实时定量PCR，检测SCAP及PDLSCs成骨能力；实时定量PCR检测SCAP及PDLSCs牙本质基质蛋白-1（dentin matrix protein-1，DMP-1）、牙本质涎磷蛋白（dentin sialophosphoprotein，DSPP）的表达。结果流式细胞仪检测结果显示两种细胞CD105、CD90及CD146表达阳性；CCK-8法结果显示SCAP增殖活力高于PDLSCs（P<0.05）；茜素红染色结果显示，与PDLSCs相比，SCAP矿化结节增多；qRT-PCR结果显示，SCAP骨桥蛋白（Osteopontin, OPN）、Runt相关转录因子2（Runt-related transcription factor 2, Runx2）较PDLSCs表达量升高（P<0.05）；牙本质基质蛋白-1（dentin matrix protein-1，DMP-1）、牙本质涎磷蛋白（dentin sialophosphoprotein，DSPP）同样较PDLSCs表达水平高（P<0.05）。结论根尖乳头干细胞与牙周膜干细胞相比有较强的增殖、成骨及成牙本质能力。

关键词: 牙周膜干细胞, 根尖乳头干细胞, 分化

Abstract: Objective　To compare the proliferation, osteogenic and dentinogenic differentiation ability of stem cells from apical papilla and periodontal ligament stem cells, this study provides a theoretical basis for the application of stem cells in root regeneration. Methods PDLSCs and SCAP were obtained by combining enzyme digestion method and tissue block method. The expressions of stem cell surface markers CD90, CD146 were detected by FACS. The proliferation abilities of PDLSCs and SCAP were evaluated by CCK-8 method. The osteogenic abilities of PDLSCs and SCAP were tested by alizarin red staining and quantitative real-time PCR. And the expressions of dentinogenic protein: dentin matrix protein-1(DMP-1), dentin sialophosphoprotein(DSPP) were detected by quantitative real-time PCR. Result FACS profiling showed that PDLSCs and SCAP were strongly positive for CD105、CD90 and CD146. The results of CCK-8 showed that SCAP could promote the proliferation of PDLSCs (P<0.05). The alizarin red staining showed that the SCAP of mineralized nodules increased . Quantitative real-time PCR results showed that the expression of osteogenic marker genes Cementum attachment protein(CAP), Osteopontin(OPN), Runt-related transcription factor 2(RUNX-2) in SCAP were significantly higher than PDLSCs(P<0.05), and the expression levels of DMP-1 and DSPP in SCAP were significantly higher than PDLSCs (P<0.05). Conclusion The apical papilla stem cells have stronger proliferation, osteogenic and dentinogenic ability than periodontal ligament stem cells.

Key words: Stem cells from apical papilla, Periodontal ligament stem cells, Differentiation

中图分类号:

R781.3

马玉段子文李淑慧吴佩玲. 牙周膜干细胞与根尖乳头干细胞成骨及成牙本质能力比较的实验研究[J]. , 2018, 38(1): 5-9.

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