›› 2018, Vol. 38 ›› Issue (6): 503-507.

• 基础研究 • 上一篇    下一篇

牙龈卟啉单胞菌刺激树突状细胞激活炎症反应

宋亮1,徐斌1,刘阳2,刘柳慧1,郑义彩3,肖国岫1,施晓军1   

  1. 1. 复旦大学附属上海市第五人民医院
    2. 复旦大学生物医学研究院
    3. 复旦大学附属上海市第五人民医院口腔科
  • 收稿日期:2017-11-13 修回日期:2017-12-28 出版日期:2018-06-28 发布日期:2018-07-05
  • 通讯作者: 宋亮 E-mail:roseman7777@163.com
  • 基金资助:
    上海市卫生局课题;上海市闵行区科委课题;复旦大学附属上海市第五人民医院菁英人才培养项目

Porphyromonas gingivalis stimulates dendritic cells to activate inflammatory responses

  • Received:2017-11-13 Revised:2017-12-28 Online:2018-06-28 Published:2018-07-05

摘要: [摘要] 目的 分析树突状细胞在牙龈卟啉单胞菌感染后的差异表达基因,探讨牙周炎的致病机制及为日后研究提供线索。方法 利用高通量测序技术来分析体外诱导分化的树突状细胞在牙龈卟啉单胞菌感染情况下的差异表达基因、基因集及相关功能。 结果 本实验总计发现7305个至少有两倍变化的差异表达基因。基因本体结果显示牙龈卟啉单胞菌感染后树突状细胞的生物过程、细胞成份、分子功能均发生了较大变化。基因集富集分析结果显示肿瘤坏死因子基因集、细胞因子及炎症反应基因集、白介素1及转录因子NF-kB家族基因集、干扰素信号基因集、B细胞受体信号基因集、T细胞受体信号基因集富集明显。结论 树突状细胞在牙龈卟啉单胞菌感染后会导致基因水平的变化,引起机体免疫反应及炎症反应的发生, CXCL及SOCS的水平及作用可作为日后研究的方向。

关键词: 牙龈卟啉单胞菌, 树突状细胞, 牙周炎, 免疫反应

Abstract: Abstract: Objective To analyze differentially expressed genes of dendritic cells infected with Porphyromonas gingivalis and explore the pathogenesis of periodontitis and to provide clues for future research. Methods High-throughput sequencing was used to analyze differentially expressed genes, gene pools and related functions of in vitro induced dendritic cells under P. gingivalis infection. Results A total of 7305 differentially expressed genes with at least two-fold changes were found in this experiment. The results of gene ontology showed that the biological processes, cell components, and molecular functions of dendritic cells after P. gingivalis infection greatly changed. Gene enrichment analysis showed that the tumor necrosis factor gene set, cytokine and inflammatory response gene set, interleukin 1 and NF-kB family of gene transcription factor, interferon signal gene set, B cell receptor signal gene set, T cell receptor signal gene set enriched significantly. Conclusions Dendritic cells can cause genetic changes after P. gingivalis infection, causing the immune response and inflammatory reaction. The levels and effects of CXCL and SOCS could be the direction of future research.

Key words: Porphyromonas gingivalis, Dendritic cells, Periodontitis, Immune response

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