›› 2018, Vol. 35 ›› Issue (9): 774-779.

• 基础研究 • 上一篇    下一篇

Ptk1和SinR对牙龈卟啉单胞菌细胞外多糖形成的影响

张盛丹1,牛毓龙2,丁一1,刘程程1   

  1. 1. 口腔疾病研究国家重点实验室,四川大学华西口腔医院牙周病科
    2. 四川大学生命科学学院,生物资源与生态环境教育部重点实验室
  • 收稿日期:2018-04-26 修回日期:2018-05-20 出版日期:2018-09-28 发布日期:2018-09-14
  • 通讯作者: 刘程程 E-mail:liuchengcheng519@163.com
  • 基金资助:
    国家自然科学基金

Effect of Ptk1 and SinR on polysaccharide production of Porphyromonas gingivalis

  • Received:2018-04-26 Revised:2018-05-20 Online:2018-09-28 Published:2018-09-14

摘要: 目的 探究牙龈卟啉单胞菌(Porphyromonas gingivalis,P. gingivalis)转录因子SinR和酪氨酸激酶Ptk1对P. gingivalis细胞外多糖形成的影响及其上下游调控关系。方法 利用重组PCR技术,构建P. gingivalis ATCC 33277 sinR单基因缺失突变菌株(ΔsinR)以及ptk1/sinR双基因缺失突变菌株(Δptk1/sinR),通过激光共聚焦显微镜检测Δptk1、ΔsinR和Δptk1/sinR菌株细胞外多糖的形成情况。结果 与野生菌株P. gingivalis 33277相比,P. gingivalis Δptk1菌株细胞外多糖合成显著减少(P<0.05),P. gingivalis ΔsinR菌株细胞外多糖合成显著增加(P<0.05),而Δptk1/sinR菌株细胞外多糖合成明显减少(P<0.05)。结论 SinR和Ptk1对P. gingivalis细胞外多糖合成具有显著调控作用,并且Ptk1对P. gingivalis细胞外多糖的调控作用处于SinR的下游。

关键词: 牙龈卟啉单胞菌, 细胞外多糖, Ptk1, SinR

Abstract: Objective To investigate the mechanism of Porphyromonas gingivalis (P. gingivalis) transcription factor SinR and tyrosine kinase Ptk1 on the production of exopolysaccharide and the regulative relation between upstream and downstream. Methods The PCR fusion technique was employed to generate allelic exchange mutants of sinR (ΔsinR) and ptk1/sinR double deletion mutant (Δptk1/sinR) in ATCC 33277 . The exopolysaccharide production of Δptk1、ΔsinR and Δptk1/sinR strain of P. gingivalis with fluorescent lectins was detected by laser scanning confocal microscope (CLSM). Results Polysaccharide production of Δptk1 and Δptk1/sinR significantly decreased(P<0.05), while that of ΔsinR significantly increased, compared with wild strain P. gingivalis 33277(P<0.05). Conclusion These data suggest that SinR and Ptk1 have significant regulatory effects on of exopolysaccharide synthesis in P.gingivalis. In addition, Ptk1 functioned downstream of SinR for exopolysaccharide production in P. gingivalis.

Key words: P.gingivalis, extracellular polysaccharides, Ptk1, SinR

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