›› 2019, Vol. 39 ›› Issue (9): 785-788.

• 基础研究 • 上一篇    下一篇

阿奇霉素对牙周炎大鼠牙槽骨影响的Micro-CT研究

盛敏,石宁   

  1. 北京老年医院
  • 收稿日期:2018-06-11 修回日期:2018-07-20 出版日期:2019-09-28 发布日期:2019-09-17
  • 通讯作者: 盛敏 E-mail:shengmi9863@sina.com

Micro-CT study on the effect of azithromycin on alveolar bone in rats with periodontitis

  • Received:2018-06-11 Revised:2018-07-20 Online:2019-09-28 Published:2019-09-17
  • Contact: MIN Sheng E-mail:shengmi9863@sina.com

摘要: 目的 通过MicroCT研究阿奇霉素对牙周炎大鼠牙槽骨微结构的影响。 方法 雄性SD大鼠随机分为3组:对照组、结扎组、阿奇霉素组。结扎组和阿奇霉素组大鼠均采用丝线结扎左侧上颌第一磨牙,建立实验性牙周炎模型。从结扎起阿奇霉素组大鼠每天腹腔注射阿奇霉素3.5 mg/kg。注射2周后,将大鼠处死,取上颌骨。采用MicroCT分析大鼠上颌第一磨牙牙槽骨吸收和微结构的改变。 结果 结扎组与对照组比较上颌第一磨牙周围牙槽骨的高度明显减少,而阿奇霉素组在药物诱导下牙槽骨吸收明显减小,阿奇霉素对牙槽骨高度降低起抑制作用。大鼠结扎后,牙槽骨微结构发生明显改变,其中BV/TV、Tb.N、Tb.Th和BMD值均明显降低,而Tb.Sp值增加。而阿奇霉素组牙槽骨微结构参数能恢复到一定水平,与对照组的差异明显减少。 结论 阿奇霉素可阻止实验性大鼠牙周炎的牙槽骨继续破坏,对实验性大鼠牙周炎有治疗作用。

关键词: 阿奇霉素, 实验性牙周炎, 牙槽骨, Micro-CT, SD大鼠

Abstract: Objective To study the effects of azithromycin on the micro structure of alveolar bone of rats with periodontitis by MicroCT. Methods Male SD rats were randomly divided into 3 groups:control group, ligation group, azithromycin group. The left maxillary first molars of rats in ligation group and the azithromycin group were ligated by thread to establish the experimental periodontitis model. From the ligation, rats in azithromycin group received intraperitoneal injection of azithromycin 3.5 mg/kg. After two weeks’ injection, the rats were killed and the maxillary bones were removed. By MicroCT the changes of maxillary first molar alveolar bone loss and microstructure were analyzed. Results Compared with the control group, the height of the maxillary first molar surrounding alveolar bone in ligation group decreased obviously, while in drug induced state, alveolar bone resorption of azithromycin group decreased significantly. Azithromycin inhibited the alveolar bone height decrease. After ligation, micro structure of rats’ alveolar bone obviously changed. The values of BV /TV, Tb.N, Tb.Th and BMD significantly decreased, while that of Tb.Sp increased. And the alveolar bone microstructure parameters of azithromycin group could reach a certain level of recovery, and the difference with the control group decreased significantly. Conclusion Azithromycin can prevent alveolar bone of experimental periodontitis rats from continuing to be destroyed, which has a therapeutic effect on experimental periodontitis rats. Key words: azithromycin; experimental periodontitis; alveolar bone; MicroCT; SD rats

Key words: azithromycin, experimental periodontitis, alveolar bone, Micro-CT, SD rats

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