DKK-1在大鼠牙囊细胞体外的表达作用研究

›› 2019, Vol. 39 ›› Issue (10): 902-906.

DKK-1在大鼠牙囊细胞体外的表达作用研究

颜露¹,袁晓娟¹,左婕¹,盛丽¹,刘奕杉²

1. 新疆医科大学第一附属医院
2. 新疆乌鲁木齐市新市区新疆医科大学第一附属医院

收稿日期:2019-04-12 修回日期:2019-06-04 出版日期:2019-10-28 发布日期:2019-10-28
通讯作者: 刘奕杉 E-mail:lys-tree@126.com
基金资助:
国家自然科学基金

In vitro study of the effects of DKK-1 on the dental follicle cells

Lu YAN ², ², ²,

Received:2019-04-12 Revised:2019-06-04 Online:2019-10-28 Published:2019-10-28
Supported by:
National Natural Science Foundation of China

摘要/Abstract

摘要： ［摘要］目的观察Wnt/β-catenin通路的抑制因子DKK-1(Dickkopf-1)在牙囊细胞中的表达，探究在牙囊细胞成骨向分化形成过程中DKK-1的特异性表达机制，探讨其与牙齿萌出和颌骨生长发育的相关性。方法采用酶消化法分离培养牙囊细胞并纯化细胞。将牙囊细胞分为两组，对照组：普通α-MEM培养基培养，实验组：α-MEM+成骨诱导液培养。分别培养5d后，采用免疫细胞化学法观察DKK-1的表达分布；分别培养10d、20d后采用Western Blot检测DKK-1、β-catenin、Runx2的蛋白表达水平。结果免疫组织化学显示DKK-1在实验组表达较对照组减弱。Western Blot检测结果示实验组Wnt通路的关键因子DKK-1条带蛋白表达明显下调，β-catenin蛋白及成骨相关蛋白Runx2的表达上调，培养时间越长实验结果与对照组区别越明显。结论Wnt通路DKK-1在牙囊细胞中有表达且受成骨分化影响，体现Wnt/β-catenin通路中DKK-1的抑制作用，研究结果将为牙齿萌出和颌骨生长发育形成机制提供实验依据。

关键词: [关键词] DKK-1, 牙囊细胞, Wnt/β-catenin, 成骨细胞, 破骨细胞

Abstract: Abstract： Objective To study the regulating protein of Wnt signaling pathway, DKK-1, inhibitor can be expressed in the dental follicle cells. To explore the specific expression mechanism of DKK-1 during osteogenic differentiation of dental follicle cells and the relationship between the formation of mature osteoblasts and eruption of the teeth. Methods The dental follicle cells were primarily cultured by digestion method and purified. The cells were divided into 2 groups: the control group was cultured in α-MEM medium, and the experimental group was cultured in α-MEM medium supplemented with osteogenesis induction. With increased of time after intervention two groups of cells were identified using immunocytochemistry. Western-blotting was used to evaluate the lever of cellular DKK-1、β-catenin and Runx2 protein. Results Compared with control group, the factors of DKK-1 expression of experimental group was significantly lower, the Western-blot results showed that the expression of DKK-1 were significantly lower than that of the control group, but the osteogenic factor Runx2 protein、expression was enhanced in the experimental group over time. Conclusions The expression of inhibitor DKK-1 a key regulator of Wnt pathway, is affected by osteogenesis induced differentiation in the dental follicle cells and reflect its inhibitory characteristics. To suggests the molecular basis of the formation mechanism for the development of Maxillary and mandible and eruption of the teeth.

Key words: Key words： DKK-1, the dental follicle cells, Wnt/β-catenin, Osteoblast, osteoclast

中图分类号:

R341.8

颜露袁晓娟左婕盛丽刘奕杉. DKK-1在大鼠牙囊细胞体外的表达作用研究[J]. , 2019, 39(10): 902-906.

Lu YAN. In vitro study of the effects of DKK-1 on the dental follicle cells [J]. , 2019, 39(10): 902-906.

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