原发性根尖周感染中牙龈卟啉单胞菌kgp基因多态性

摘要/Abstract

摘要： 目的　通过牙龈卟啉单胞菌（Porphyromonas gingivalis，Pg）kgp基因型在原发性根尖周感染患牙根管中的分布情况，了解kgp基因在原发性根尖周感染中的基因多态性，并分析不同基因型与临床症状的相关性。方法　临床收集190例原发性根尖周感染患者，采集感染根管细菌学样本，抽提DNA，使用Pg的16S rRNA基因的特异引物PCR检测样本中是否有Pg，然后针对Pg检出阳性样本，再使用kgp特异引物PCR获得kgp催化域片段，通过Tru I酶切kgp催化域片段确定kgp的基因型，并使用Pearson χ2方法分析所得到的实验数据。结果　在本研究所采集的190例原发性根尖周感染样本中，共检出Pg55例，其中kgpⅠ型检出率为27.3%， kgp Ⅱ型检出率为38.2%。Pg的检出与临床症状有相关性；kgpⅠ型与疼痛具有相关性。结论　原发性根尖周感染的根管中Pgkgp基因存在基因多态性。因为有些样本中Pg的量较少，本研究中kgp特异引物PCR的方法难以检测到kgp基因，所以今后需发展更灵敏的检测手段以对感染根管中kgp基因做进一步的研究。

关键词: 牙龈卟啉单胞菌, kgp基因, 根尖周感染, 基因多态性

Abstract: Objective　 This study intends to investigate the distribution of Porphyromonas gingivalis and Porphyromonas gingivalis’ two kgp gene type in primary periapical infection root canal, and to analyze the correlation between the genotypes and clinical symptoms. Methods　 We collect 190 cases of primary periapical infection patients in clinic. We collect sample from their infected root cannel and we extract bacteria DNA from the sample we collected. We use Polymerase Chain Reaction to determine the presence of Porphyromonas gingivalis. We get the kgp catalytic domain with the help of Polymerase Chain Reaction. Tru I enzyme digestion to the kgp catalytic domain help us to determine the Porphyromonas gingivalis gene polymorphism. After all, we use statistical methods to the analysis of the experimental data. Results　55 patients in 190 patients with primary periapical infection can be detected with Porphyromonas gingivalis. The detection rate of kgp Itype is 27.3%, while the detection rate of kgpⅡtype is 38.2%. Porphyromonas gingivalis were correlated with clinical symptoms. kgp I type were correlated with pain. Conclusions　 The kgp gene of Porphyromonas gingivalis which is in root cannel of primary periapical infection has gene polymorphism. Because some samples do not contain enough quantity of Porphyromonas gingivalis, it is sometimes difficult for method of kgp specific primers PCR to detect kgp gene. In further research, we will find more sensitive means to detect kgp gene in infected root canals.

Key words: Porphyromonas gingivalis, kgp gene, gene polymorphism, periapical infection

中图分类号:

R781.3

毛骁俊漆正楠沈妙莲王畅何剑亮诸晓丹邹岩唐子圣. 原发性根尖周感染中牙龈卟啉单胞菌kgp基因多态性[J]. , 2019, 39(11): 1003-1008.

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