四取代羧酸酞菁锌光动力诱导口腔鳞状细胞癌细胞凋亡

›› 2019, Vol. 39 ›› Issue (4): 289-295.

四取代羧酸酞菁锌光动力诱导口腔鳞状细胞癌细胞凋亡

罗亚东¹,丁旭¹,杜洪明²,宋海洋³,马虞楠²,李萌²,武和明¹

1. 南京医科大学附属口腔医院
2. 南京医科大学口腔医学研究所南京医科大学附属口腔医院口腔颌面外科
3. 南京医科大学

收稿日期:2018-10-18 修回日期:2018-12-20 出版日期:2019-04-28 发布日期:2020-01-13
通讯作者: 武和明 E-mail:wuheming2018@aliyun.com
基金资助:
国家自然科学基金;江苏省医学创新团队

Zinc phthalocyanines tetrasubstituted with carboxyl moieties induce cell apoptosis in oral squamous cell carcinoma cells by photodynamic therapy

Received:2018-10-18 Revised:2018-12-20 Online:2019-04-28 Published:2020-01-13
Contact: Heming Wu E-mail:wuheming2018@aliyun.com
Supported by:
National Natural Science Foundation of China;Jiangsu Provincial Medical Innovation Team

摘要/Abstract

摘要： 目的探讨光敏剂四取代羧酸酞菁锌介导的光动力疗法(photodynamic therapy，PDT)对体外培养的口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）细胞的光动力作用。方法以人口腔鳞状细胞癌细胞系HN4为研究对象。实验分组为不同梯度浓度的暗反应组和光反应组，光反应组细胞行梯度光照剂量垂直照射处理。CCK-8法检测不同四取代羧酸酞菁锌浓度及不同光照剂量对HN4细胞增殖的影响，流式细胞仪检测其对周期及凋亡的影响。蛋白质免疫印迹法（Western blot）检测不同药物浓度下Caspase-3、Bcl-2表达的变化。结果四取代羧酸酞菁锌浓度及光照剂量均影响细胞增殖（P<0.05）。暗反应组的半数抑制浓度（IC50）显著高于光反应组（P<0.05）。一定浓度范围内，随着四取代羧酸酞菁锌药物浓度的提高，细胞周期的S期相对减少，G2/M期相对增多，细胞凋亡率也随之增加（P<0.05），Caspase-3表达随之升高（P<0.05），Bcl-2表达随之降低（P<0.05）。结论四取代羧酸酞菁锌介导的PDT是一种有效的抗癌方法，能高效诱导口腔鳞癌细胞凋亡。

关键词: 光动力疗法, 酞菁锌, 口腔鳞状细胞, 凋亡

Abstract: Objective　To investigate the photosensitization of photodynamic therapy (PDT) mediated by zinc phthalocyanines tetrasubstituted with carboxyl moieties on oral squamous cell carcinoma (OSCC) cells in vitro. Methods The human OSCC cell line HN4 was studied. The experiments were divided into dark reaction group and light reaction group and the cells in the light reaction group received vertical irradiation with gradient light dose. The effect of different light doses on cell proliferation was detected by Cell Counting kit-8 (CCK-8). The effects on cell cycle changes and apoptosis were determined with flow cytometry. The changes of Caspase-3, Bcl-2 expression were examined before and after the interference by Western-blot. Results Both drug concentration and light dose affected cell proliferation (P<0.05). The 50% inhibition concentration (IC50) in the dark reaction group was significantly higher than that in the light reaction groups (P<0.05). Within the certain concentration range, with the improvement of drug concentration, the S phase of the cell cycle relatively decreased, the G2/M phase relatively increased, the number of cell apoptosis increased, the Caspase-3 expression increased while the Bcl-2 expression decreased (P<0.05). Conclusion Zinc phthalocyanines tetrasubstituted with carboxyl moieties is an effective anti-cancer drug, which can effectively induce the apoptosis of OSCC.

Key words: photodynamic therapy, zinc phthalocyanine, oral squamous cell carcinoma cells, cell apoptosis.

中图分类号:

R739.8

罗亚东丁旭杜洪明宋海洋马虞楠李萌武和明. 四取代羧酸酞菁锌光动力诱导口腔鳞状细胞癌细胞凋亡[J]. , 2019, 39(4): 289-295.

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