微弧氧化法制备不同浓度含锌涂层表面特征及抗菌性能的研究

›› 2019, Vol. 39 ›› Issue (4): 311-315.

微弧氧化法制备不同浓度含锌涂层表面特征及抗菌性能的研究

李玥锟¹,徐琳²,杜青³,谢伟丽⁴

1. 哈尔滨医学大学附属第一医院
2. 哈尔滨医科大学口腔医学院修复科
3. 哈尔滨工业大学
4. 哈尔滨医科大学附属口腔医院

收稿日期:2018-07-31 修回日期:2018-08-29 出版日期:2019-04-28 发布日期:2020-01-13
通讯作者: 谢伟丽 E-mail:xwl811@126.com
基金资助:
黑龙江省科技攻关项目

Study on Surface Characteristics and Antibacterial Properties of Different Concentration Zinc Coatings Prepared by Microarc Oxidation

Received:2018-07-31 Revised:2018-08-29 Online:2019-04-28 Published:2020-01-13

摘要/Abstract

摘要： 目的用微弧氧化法在纯钛表面制备不同浓度含锌涂层，比较其表面形貌、元素分布及抗菌性能。方法实验共分5组，抛光后纯钛作为对照组（CP组），未添加锌为无锌组（N-Zn组），根据引入不同浓度锌分为低锌组（L-Zn组）、中锌组（M-Zn组）、高锌组（H-Zn组）；扫描电镜及能谱仪观察各组涂层表面形貌、元素分布；贴膜法测定涂层对大肠杆菌和金黄色葡萄球菌的抑制效果。结果 ①扫描电镜观察：CP组表面无孔洞结构，成沟槽形，4组实验组表面出现空洞结构，呈火山口样，N-Zn组、L-Zn组组表面纳米孔直径200~300 nm，M-Zn组表面纳米孔直径100~200 nm，H-Zn组表面纳米孔直径50~100 nm；②能谱仪显示，与CP组、N-Zn组相比，L-Zn、M-Zn、H-Zn组涂层表面成功引入了锌元素，且原子比例依次增高，具有显著统计学差异（P<0.01）；③贴膜法实验结果：各组间菌落数相比，具有显著统计学差异（P<0.01）。结论微弧氧化法制备的含锌涂层具有抗菌性能，抗菌性能随锌离子浓度升高而增强。

关键词: 微弧氧化, 锌, 抗菌

Abstract: PURPOSE：Prepare zinc-containing coatings with different concentrations on pure titanium by micro-arc oxidation, and compare the surface morphology, element distribution and antibacterial properties. METHODS: The experiment was divided into 5 groups. Pure titanium after polishing was used as the control group (group A), Without added zinc was used as zinc-free group(group B), according to the introduction of different concentrations of zinc was divided into low-zinc group (group C)、medium-zinc group(Group D) and high-zinc group (Group E); SEM and EDS were used to observe the surface morphologyand element distribution of each group; Film coating method was used to test the inhibitory effect of Escherichia coli and Staphylococcus aureus.RESULTS: ①SEM observation: There was no pore structure on the surface of Group A, and troughs were formed. The voids on the surface of the four experimental groups showed a crater-like structure. The diameter of the nanopore on the surface of group B and C is 200-300nm, the diameter of the nanopore on the surface of group D is 100-200nm, and the diameter of nanopore on the surface of group E is 50-100nm. ② EDS showed that compared with group A and group B, zinc was successfully introduced on the surface of the coating in groups C, D, and E, and the atomic ratio increased in turn, there was a significant statistical difference(P<0.01)；③Film coating test results: There was a statistically significant difference in the number of colonies between the groups (P<0.01).Conclusion: The zinc-containing coating prepared by micro-arc oxidation has antibacterial properties, and the antibacterial property increases with the increase of zinc ion concentration.

Key words: micro-arc oxidation, zinc, antibacterial

中图分类号:

R783.1

李玥锟徐琳杜青谢伟丽. 微弧氧化法制备不同浓度含锌涂层表面特征及抗菌性能的研究[J]. , 2019, 39(4): 311-315.

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