脱细胞外基质支架材料在骨再生中的应用及研究进展

摘要/Abstract

摘要： [摘要] 近年来，因肿瘤、创伤、发育畸形、感染等原因造成骨缺损的修复材料包括自体骨或异体骨、人工合成材料、生物活性材料。然而，这些材料因取骨量限制、免疫排斥、成骨活性低、支架降解等问题一直困扰着临床医生。理想的骨缺损材料应具备三个要素,募集种子细胞、缓释生长因子、提供模拟微环境的支架。随着骨组织工程不断发展，脱细胞外基质材料（decellularuzed Extracellular Matrix,dECM）作为生物活性支架材料因其无免疫源性、良好的成骨性能、缓释生长因子、促进种子细胞的粘附、干性的维持等优点而倍受关注。本文围绕脱细胞外基质材料（dECM）在骨再生中的应用及研究进展作一综述。

关键词: [关键词] 细胞外基质材料, 骨再生, 脱细胞

Abstract: Abstract: Recently,the restoration of bone defect，caused by tumor、trauma、malformation、infection and so on,include auto or allogenic bone、synthetic material and bioactive material.However,the mentioned above materials are still bothering clinicians due to its limited acquired bone volume、immunological rejection、lower osteogenic property and scaffold degradation.And the perfect biomaterial should equip the following three key factors:recruiting seeding cell、slow-releasing growth factors、and serving as mimicking microenvironment scaffold.With the rapidly advancement of bone engineering,dcellularized extracellular matrix(dECM) as bioactive scaffold materials,attributed to freeing of immunogenicity、ideal osteogenic property、slowing-releasing growth factors、promoting adhesion of seeding cell and even maintaining stem property of seeding cell,is arousing widely interests.This paper maks a review aiming at the application and research progress of decellularized extracellular matrix scaffold material in bone regeneration.

Key words: Key words: dECM, bone regeneration, dcellularization

中图分类号:

R782.2

张艳珉杨国利姜治伟王慧明. 脱细胞外基质支架材料在骨再生中的应用及研究进展[J]. 口腔医学, 2020, 40(1): 63-66.

参考文献 40

[1]	BOSE S, ROY M, BANDYOPADHYAY A J T I B.Recent advances in bone tissue engineering scaffolds[J].Trends in Biotechnology, 2012, 30(10):546-554
[2]	CAI R, NAKAMOTO T, HOSHIBA T, et al.Matrices secreted during simultaneous osteogenesis and adipogenesis of mesenchymal stem cells affect stem cells differentiation[J].Acta Biomaterialia, 2016, 35(15):185-193
[3]	PREWITZ M C, STISSEL A, FRIEDRICHS J, et al.Extracellular matrix deposition of bone marrow stroma enhanced by macromolecular crowding [J]. Biomaterials, 2015, 73:60-69.[J].Biomaterials, 2015, 73:60-69
[4]	FARAG A, VAQUETTE C, THEODOROPOULOS C, et al.Decellularized periodontal ligament cell sheets with recellularization potential[J].Journal of Dental Research, 2014, 93(12):1313-1319
[5]	BAE S E, BHANG S H, KIM B S, et al.Self-assembled extracellular macromolecular matrices and their different osteogenic potential with preosteoblasts and rat bone marrow mesenchymal stromal cells[J].Biomacromolecules, 2012, 13(9):2811-2820
[6]	ZHANG W, ZHU Y, LI J, et al.Cell-derived extracellular matrix: basic characteristics and current applications in orthopedic tissue engineering[J].Tissue Engineering Part B, Reviews, 2016, 22(3):193-207
[7]	LIN X, ZHAO C, ZHU P, et al.Periosteum extracellular-matrix-mediated acellular mineralization during bone formation[J].Advanced Healthcare Materials, 2018, 7(4):1700660-
[8]	ZHANG X, LI H, SUN J, et al.Cell-derived micro-environment helps dental pulp stem cells promote dental pulp regeneration[J].Cell Proliferation, 2017, 50(5):e12361-
[9]	CRAPO P M, GILBERT T W, BADYLAK S F.An overview of tissue and whole organ decellularization processes[J].Biomaterials, 2011, 32(12):3233-3243
[10]	GILBERT T W.Strategies for tissue and organ decellularization[J].Journal of Cellular Biochemistry, 2012, 113(7):2217-2222
[11]	ANTEBI B, ZHANG Z, WANG Y, et al.Stromal-cell-derived extracellular matrix promotes the proliferation and retains the osteogenic differentiation capacity of mesenchymal stem cells on three-dimensional scaffolds[J].Tissue Engineering Part C, Methods, 2015, 21(2):171-181
[12]	SONG J S, TAKIMOTO K, JEON M, et al.Decellularized human dental pulp as a scaffold for regenerative endodontics[J].Journal of Dental Research, 2017, 96(6):640-646
[13]	XIONG Y, HE J, ZHANG W, et al.Retention of the stemness of mouse adipose-derived stem cells by their expansion on human bone marrow stromal cell-derived extracellular matrix[J].Tissue Engineering Part A, 2015, 21(11-12):1886-1894
[14]	ZHANG Z, LUO X, XU H, et al.Bone marrow stromal cell-derived extracellular matrix promotes osteogenesis of adipose-derived stem cells[J].Cell Biology International, 2015, 39(3):291-299
[15]	WANG X, CHEN Z, ZHOU B, et al.Cell-Sheet-Derived ECM coatings and their effects on BMSCs responses[J].ACS Applied Materials & Interfaces, 2018, 10(14):11508-11518
[16]	SUN Y, LI W, LU Z, et al.Rescuing replication and osteogenesis of aged mesenchymal stem cells by exposure to a young extracellular matrix[J].FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2011, 25(5):1474-1485
[17]	LIN H, YANG G, TAN J, et al.Influence of decellularized matrix derived from human mesenchymal stem cells on their proliferation,migration and multi-lineage differentiation potential[J].Biomaterials, 2012, 33(18):4480-4489
[18]	MOTOIKE S, KAJIYA M, KOMATSU N, et al.Cryopreserved clumps of mesenchymal stem cellextracellular matrix complexes retain osteogenic capacity and induce bone regeneration[J].Stem Cell Research, 2018, 9(1):73-
[19]	DU P, SUBBIAH R, PARK J H, et al.Vascular morphogenesis of human umbilical vein endothelial cells on cell-derived macromolecular matrix microenvironment[J].Tissue Engineering Part A, 2014, 20(17-18):2365-2377
[20]	WEI W, LI J, CHEN S, et al.In vitro osteogenic induction of bone marrow mesenchymal stem cells with a decellularized matrix derived from human adipose stem cells and in vivo implantation for bone regeneration[J].Journal of Materials Chemistry B, 2017, 5(13):2468-2482
[21]	KIM J, MA T J B P.Endogenous extracellular matrices enhance human mesenchymal stem cell aggregate formation and survival[J].Biotechnology Progress, 2013, 29(2):441-451
[22]	KIM I G, HWANG M P, DU P, et al.Bioactive cell-derived matrices combined with polymer mesh scaffold for osteogenesis and bone healing [J]. Biomaterials, 2015, 50:75-86.[J].Biomaterials, 2015, 50:75-86
[23]	HARVESTINE J N, VOLLMER N L, HO S S, et al.Extracellular matrix-coated composite scaffolds promote mesenchymal stem cell persistence and osteogenesis[J].Biomacromolecules, 2016, 17(11):3524-3531
[24]	PATI F, SONG T H, RIJAL G, et al.Ornamenting 3D printed scaffolds with cell-laid extracellular matrix for bone tissue regeneration [J]. Biomaterials, 2015, 37:230-241.[J].Biomaterials, 2015, 37:230-241
[25]	ONISHI T, SHIMIZU T, AKAHANE M, et al.Osteogenic extracellular matrix sheet for bone tissue regeneration [J]. European Cells & Materials, 2018, 36:68-80.[J].European Cells & Materials, 2018, 36:68-80
[26]	YANG Y, LIN H, SHEN H, et al.Mesenchymal stem cell-derived extracellular matrix enhances chondrogenic phenotype of and cartilage formation by encapsulated chondrocytes in vitro and in vivo[J].Acta Biomaterialia, 2018, 69(15):71-82
[27]	LI M, CHEN X, YAN J, et al.Inhibition of osteoclastogenesis by stem cell-derived extracellular matrix through modulation of intracellular reactive oxygen species[J].Acta Biomaterialia, 2018, 71(15):118-131
[28]	ZHOU L, CHEN X, LIU T, et al.SIRT1-dependent anti-senescence effects of cell-deposited matrix on human umbilical cord mesenchymal stem cells[J].Journal of Tissue Engineering and Regenerative Medicine, 2018, 12(2):e1008-e1021
[29]	SCHULTZ H S, NITZE L M, ZEUTHEN L H, et al.Collagen induces maturation of human monocyte-derived dendritic cells by signaling through osteoclast-associated receptor[J].Journal of Immunology (Baltimore, Md), 2015, 194(7):3169-3179
[30]	SALBACH-HIRSCH J, ZIEGLER N, THIELE S, et al.Sulfated glycosaminoglycans support osteoblast functions and concurrently suppress osteoclasts[J].Journal of Cellular Biochemistry, 2014, 115(6):1101-1111
[31]	HOCH A I, MITTAL V, MITRA D, et al.Cell-secreted matrices perpetuate the bone-forming phenotype of differentiated mesenchymal stem cells [J]. Biomaterials, 2016, 74:178-187.[J].Biomaterials, 2016, 74:178-187
[32]	JANSON I A, PUTNAM A J.Extracellular matrix elasticity and topography: material-based cues that affect cell function via conserved mechanisms[J].Journal of Biomedical Materials Research Part A, 2015, 103(3):1246-1258
[33]	MOSQUEIRA D, PAGLIARI S, UTO K, et al.Hippo pathway effectors control cardiac progenitor cell fate by acting as dynamic sensors of substrate mechanics and nanostructure[J].ACS Nano, 2014, 8(3):2033-2047
[34]	KEGELMAN C D, MASON D E, DAWAHARE J H, et al.Skeletal cell YAP and TAZ combinatorially promote bone development[J].FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2018, 32(5):2706-2721
[35]	LARJAVA H, KOIVISTO L, HAKKINEN L, et al.Epithelial integrins with special reference to oral epithelia[J].Journal of Dental Research, 2011, 90(12):1367-1376
[36]	RAHMANY M B, VAN DYKE M.Biomimetic approaches to modulate cellular adhesion in biomaterials: A review[J].Acta Biomaterialia, 2013, 9(3):5431-5437
[37]	LECHT S, STABLER C T, RYLANDER A L, et al.Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells[J].Biomaterials, 2014, 35(10):3252-3262
[38]	OLIVARES-NAVARRETE R, HYZY S L, PARK J H, et al.Mediation of osteogenic differentiation of human mesenchymal stem cells on titanium surfaces by a Wnt-integrin feedback loop[J].Biomaterials, 2011, 32(27):6399-6411
[39]	SUN M, CHI G, XU J, et al.Extracellular matrix stiffness controls osteogenic differentiation of mesenchymal stem cells mediated by integrin alpha5[J].Stem Cell Research & Therapy, 2018, 9(1):52-
[40]	KEANE T J, LONDONO R, TURNER N J, et al.Consequences of ineffective decellularization of biologic scaffolds on the host response[J].Biomaterials, 2012, 33(6):1771-1781