LncRNA调控PDLSCs成骨分化的研究进展

摘要/Abstract

摘要： ［摘要］牙周膜干细胞（periodontal ligament stem cells, PDLSCs）是一种间充质干细胞,是牙周组织改建和修复的种子细胞。正常条件下,PDLSCs成骨分化功能稳定,维持骨组织成骨和破骨的动态平衡。然而炎症环境的刺激导致PDLSCs分化能力受损,造成牙周组织丧失且无法自行恢复。恢复PDLSCs的再生能力是牙周再生的一个重要途径。长链非编码RNA（Long non-coding RNA,LncRNA）可以从多种途径参与炎症反应调控,并且与PDLSCs的成骨分化相关,逐渐成为研究的热点。本文就LncRNA调控PDLSCs成骨分化的机制进行综述。

关键词: 牙周膜干细胞, 长链非编码RNA, 成骨分化, 牙周再生

Abstract: Periodontal ligament stem cells（PDLSCs） are a type of mesenchymal stem cells (MSCs),which are seed cells of periodontal reconstruction and repair．In normal, the osteogenic differentiation capacity of PDLSCs is stable, maintaining the dynamic balance of osteogenesis and osteoclasia. However, the differentiation capacity of PDLSCs can be destroyed in the inflammatory environment, leading to loss of periodontal tissue, which can’t recover spontaneously. Promoting the regeneration capacity of PDLSCs is an important way of periodontal regeneration. Long non-coding RNA(LncRNA) can participate in the inflammatory response in several aspects and be related to the osteogenic differentiation of PDLSCs，and increasingly become a hot spot of research. This article reviewed the mechanism of LncRNA regulating the osteogenic differentiation of PDLSCs.

Key words: LncRNA, PDLSCs, Osteogenic differentiation, Periodontal regeneration

中图分类号:

R781.42

孙唯夫刘佳金作林. LncRNA调控PDLSCs成骨分化的研究进展[J]. 口腔医学, 2020, 40(5): 461-464.

参考文献 40

[1]	Seo B M, Miura M, Gronthos S, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament [J]. Lancet, 2004, 364(9429): 149-155.
[2]	Akiyama K, Chen C, Gronthos S, et al. Lineage differentiation of mesenchymal stem cells from dental pulp, apical papilla, and periodontal ligament [J]. Methods in molecular biology, 2012, 887: 111-121.
[3]	Zhu B, Liu W, Zhang H, et al. Tissue-specific composite cell aggregates drive periodontium tissue regeneration by reconstructing a regenerative microenvironment [J]. Journal of tissue engineering and regenerative medicine, 2017, 11(6): 1792-1805.
[4]	Liu N, Shi S, Deng M, et al. High levels of beta-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway [J]. Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research, 2011, 26(9): 2082-2095.
[5]	Wang L, Wu F, Song Y, et al. Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients [J]. Cell Death & Disease, 2016, 7(8): e2327-e2327.
[6]	Lee J H, Pryce B A, Schweitzer R, et al. Differentiating zones at periodontal ligament-bone and periodontal ligament-cementum entheses [J]. Journal of periodontal research, 2015, 50(6): 870-880.
[7]	Chen F M, Gao L N, Tian B M, et al. Treatment of periodontal intrabony defects using autologous periodontal ligament stem cells: a randomized clinical trial [J]. Stem Cell Res Ther, 2016, 7:33.
[8]	Han J, Menicanin D, Marino V, et al. Assessment of the regenerative potential of allogeneic periodontal ligament stem cells in a rodent periodontal defect model [J]. Journal of periodontal research, 2014, 49(3): 333-345.
[9]	Li J, Xu S Q, Zhang K, et al. Treatment of gingival defects with gingival mesenchymal stem cells derived from human fetal gingival tissue in a rat model [J]. Stem Cell Res Ther, 2018, 9(1): 27.
[10]	Iwata T, Yamato M, Washio K, et al. Periodontal regeneration with autologous periodontal ligament-derived cell sheets - A safety and efficacy study in ten patients [J]. Regen Ther, 2018, 9: 38-44.
[11]	Zhu W, Liang M, et al. Periodontal Ligament Stem Cells: Current Status, Concerns, and Future Prospects [J]. Stem Cells Int 2015, 2015: 972313.
[12]	孔祥伟, 叶瑞东, 刘文佳, 等. 炎症微环境中经典Wnt信号通路对牙周膜干细胞成骨分化的调控作用 [J]. 口腔生物医学, 2015, 6(03): 129-136.
[13]	Liu D, Xu J, Liu O, et al. Mesenchymal stem cells derived from inflamed periodontal ligaments exhibit impaired immunomodulation [J]. Journal of Clinical Periodontology, 2012, 39(12): 1174-1182.
[14]	He Q, Yang S, Gu X, et al. Long noncoding RNA TUG1 facilitates osteogenic differentiation of periodontal ligament stem cells via interacting with Lin28A [J]. Cell Death Dis, 2018, 9(5): 455.
[15]	Chen X, Hu C, Wang G, et al. Nuclear factor-kappaB modulates osteogenesis of periodontal ligament stem cells through competition with beta-catenin signaling in inflammatory microenvironments [J]. Cell Death Dis, 2013, 4: e51.
[16]	Liang L, Zhou W, Yang N, et al. ET-1 Promotes Differentiation of Periodontal Ligament Stem Cells into Osteoblasts through ETR, MAPK, and Wnt/beta-Catenin Signaling Pathways under Inflammatory Microenvironment [J]. Mediators Inflamm, 2016, 2016: 8467849.
[17]	Morceau F, Chateauvieux S, Gaigneaux A, et al. Long and Short Non-Coding RNAs as Regulators of Hematopoietic Differentiation [J]. Int J Mol Sci, 2013, 14(7): 14744-14770.
[18]	Yang G, Lu X, Yuan L, et al. LncRNA: a link between RNA and cancer [J]. Biochim Biophys Acta, 2014, 1839(11): 1097-1109.
[19]	Plath K, Fang J, Mlynarczykevans S, et al. Role of Histone H3 Lysine 27 Methylation in X Inactivation [J]. Science, 2003, 300(5616): 131-135.
[20]	Luo S, Lu J Y, Liu L, et al. Divergent lncRNAs Regulate Gene Expression and Lineage Differentiation in Pluripotent Cells [J]. Cell Stem Cell, 2016, 18(5): 637-652.
[21]	Carpenter S, Fitzgerald KA. Research C. Transcription of Inflammatory Genes: Long Noncoding RNA and Beyond [J]. Journal of Interferon & Cytokine Research, 2015, 35(2): 79-88.
[22]	Wang P, Xue Y, Han Y, et al. The STAT3-binding long noncoding RNA lnc-DC controls human dendritic cell differentiation [J]. Science, 2014, 344(6181): 310-313.
[23]	Zhu X, Du J, Yu J, et al. LncRNA NKILA regulates endothelium inflammation by controlling a NF-kappaB/KLF4 positive feedback loop [J]. J Mol Cell Cardiol, 2019, 126:60-69.
[24]	Li Z, Chao T C, Chang K Y, et al. The long noncoding RNA THRIL regulates TNFalpha expression through its interaction with hnRNPL [J]. Proc Natl Acad Sci USA, 2014, 111(3):1002-1007.
[25]	Qu Q, Fang F, Wu B, et al. Potential Role of Long Non-Coding RNA in Osteogenic Differentiation of Human Periodontal Ligament Stem Cells [J]. Journal of Periodontology, 2016, 87(7): e127-e137.
[26]	Zheng Y, Li X, Huang Y, et al. Time series clustering of mRNA and lncRNA expression during osteogenic differentiation of periodontal ligament stem cells [J]. PeerJ, 2018, 6: e5214.
[27]	Jia B, Qiu X, Chen J, et al. A feed-forward regulatory network lncPCAT1/miR-106a-5p/E2F5 regulates the osteogenic differentiation of periodontal ligament stem cells [J]. J Cell Physiol, 2019 ,234: 19523–19538,
[28]	Jia Q, Jiang W, Ni L. Down-regulated non-coding RNA (lncRNA-ANCR) promotes osteogenic differentiation of periodontal ligament stem cells [J]. Arch Oral Biol, 2015, 60(2): 234-241.
[29]	Peng W, Deng W, Zhang J, et al. Long noncoding RNA ANCR suppresses bone formation of periodontal ligament stem cells via sponging miRNA-758 [J]. Biochem Biophys Res Commun, 2018, 503(2): 815-821.
[30]	Zhang W, Chen L, Wu J, et al. Long noncoding RNA TUG1 inhibits osteogenesis of bone marrow mesenchymal stem cells via Smad5 after irradiation [J]. Theranostics, 2019, 9(8): 2198-2208.
[31]	Gu X, Li M, Jin Y, et al. Identification and integrated analysis of differentially expressed lncRNAs and circRNAs reveal the potential ceRNA networks during PDLSC osteogenic differentiation [J]. BMC Genet, 2017, 18: 100-113.
[32]	Li H, Li T, Wang S, et al. miR-17-5p and miR-106a are involved in the balance between osteogenic and adipogenic differentiation of adipose-derived mesenchymal stem cells [J]. Stem Cell Research, 2013, 10(3): 313-324.
[33]	Urata M, Kokabu S, Matsubara T, et al. A peptide that blocks the interaction of NF-κB p65 subunit with Smad4 enhances BMP2-induced osteogenesis [J]. Journal of Cellular Physiology, 2018, 233(9): 7356-7366.
[34]	Zou Y, Li C, Shu F, et al. lncRNA Expression Signatures in Periodontitis Revealed by Microarray: The Potential Role of lncRNAs in Periodontitis Pathogenesis [J]. Journal of Cellular Biochemistry, 2015, 116(4): 640-647.
[35]	Wang Y, Sun Y, Zheng P, et al. Long non-coding RNAs mortal obligate RNA transcript regulates the proliferation of human periodontal ligament stem cells and affects the recurrence of periodontitis [J]. Archives of Oral Biology, 2019, 105:1-4.
[36]	Zhang Q, Chen L, Cui S, et al. Expression and regulation of long noncoding RNAs during the osteogenic differentiation of periodontal ligament stem cells in the inflammatory microenvironment [J]. Scientific Reports, 2017, 7: 13991-14003.
[37]	Kim K M, Park S J, Jung S H, et al. miR-182 is a negative regulator of osteoblast proliferation, differentiation, and skeletogenesis through targeting FoxO1 [J]. Journal of Bone and Mineral Research, 2012, 27(8): 1669-1679.
[38]	Liu W, Zheng Y, Chen B, et al. LncRNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) regulates the proliferation of human periodontal ligament stem cells and toll-like receptor 4 (TLR4) expression to improve periodontitis [J]. BMC Oral Health, 2019, 19(1): 108
[39]	Xia S, Ji R, Zhan W J B N. Long noncoding RNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) inhibits proliferation and invasion of glioma cells by suppressing the Wnt/β-catenin signaling pathway [J]. BMC Neurology ,2017, 17(1): 30.
[40]	Xu Y, Qin W, Guo D, et al. LncRNA-TWIST1 Promoted Osteogenic Differentiation Both in PPDLSCs and in HPDLSCs by Inhibiting TWIST1 Expression [J]. BioMed Research International, 2019, 2019: 8735952.