S100A8在人慢性牙周炎牙龈组织中巨噬细胞表达的研究

›› 2020, Vol. 40 ›› Issue (6): 486-490.

S100A8在人慢性牙周炎牙龈组织中巨噬细胞表达的研究

刘利思¹,杨婷¹,叶展鸿²,孙澎³,王刚⁴,黄世光³

1. 暨南大学口腔医学院
2. 中山大学中山医学院
3. 暨南大学
4. 中山市人民医院

收稿日期:2019-09-18 修回日期:2019-11-20 出版日期:2020-06-28 发布日期:2020-06-28
通讯作者: 黄世光 E-mail:thshg@126.com
基金资助:
中山市社会公益科技研究专项资金立项项目

Expression of S100A8 on macrophages in gingival tissue with human chronic periodontitis

Received:2019-09-18 Revised:2019-11-20 Online:2020-06-28 Published:2020-06-28
Contact: Shiguang Huang E-mail:thshg@126.com

摘要/Abstract

摘要： [摘要] 目的观察S100钙结合蛋白A8(S100 Calcium Binding Protein A8,S100A8)在人慢性牙周炎牙龈组织中巨噬细胞（Macrophages, m?）上的表达情况，探讨S100A8在慢性牙周炎发病机制中的作用。方法将60例受试者牙龈组织标本按牙周炎的病变程度分为三组：正常对照组20例、轻度牙周炎组20例、重度牙周炎组20例。组织标本经4 %?多聚甲醛溶液固定，包埋、切片、 HE染色。各组标本在光学显微镜下观察其组织学变化；经免疫荧光双染色(?double immunofluorescence，DIF)，在荧光显微镜下观察S100A8在m?上的表达情况并计算。结果与正常对照组比较，人慢性牙周炎牙龈组织中CD68-S100A8双阳性m?表达水平均显著升高(P<0.05)；重度牙周炎组CD68-S100A8双阳性m?细胞数明显高于轻度牙周炎组（P<0.05）。结论 CD68-S100A8双阳性巨噬细胞与人慢性牙周炎的发生、发展及致病机制有关。

关键词: 慢性牙周炎, 巨噬细胞, S100钙结合蛋白A8, 免疫荧光双染色

Abstract: Abstract: Objective To observe the expression of S100 Calcium Binding Protein A8 (S100A8) on macrophages (m?) in gingival tissue with chronic periodontitis, to analyze the role of CD68-S100A8 double positive m? in the pathogenesis of chronic periodontitis. Methods The gingival tissue specimens of 60 subjects were divided into three groups according to the degree of periodontitis lesions: normal control group (20 cases), mild periodontitis group (20 cases) and severe periodontitis group (20 cases). Tissue specimens were fixed by 4% paraformaldehyde solution, stained with hematoxylin and eosin for histopathology，stained with double-immunofluorescence for identification of CD68-S100A8 double-positive m? in gingival tissues. Results Compared with the normal control group, the expression of CD68-S100A8 double positive m? in gingival tissues with human chronic periodontitis was significantly increased (P<0.05); the number of CD68-S100A8 double positive m? in severe periodontitis group was significantly higher than that in the slight periodontitis group (P < 0.05). Conclusion The results suggest that the CD68-S100A8 double positive m? may play important roles in the initiation, development and pathogenesis of the human chronic periodontitis.

Key words: chronic periodontitis, macrophages, S100 calcium binding protein A8, double immunofluorescence

中图分类号:

R781.42

刘利思杨婷叶展鸿孙澎王刚黄世光. S100A8在人慢性牙周炎牙龈组织中巨噬细胞表达的研究[J]. 口腔医学, 2020, 40(6): 486-490.

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