白色念珠菌标准株及耐药株自噬的比较研究

›› 2020, Vol. 40 ›› Issue (8): 677-681.

• 基础研究 • 下一篇

白色念珠菌标准株及耐药株自噬的比较研究

孙凯瑞¹,姜刘鎏²,周鹏²,顾静怡³,徐瑞¹,魏昕²

1. 南京医科大学附属口腔医院牙体牙髓科
2. 南京医科大学附属口腔医院
3. 南京医科大学附属江苏省口腔医院牙体牙髓科

收稿日期:2020-04-23 修回日期:2020-05-26 出版日期:2020-08-29 发布日期:2020-08-28
通讯作者: 孙凯瑞 E-mail:1627080053@qq.com
基金资助:
国家自然科学基金资助项目;江苏高校优势学科建设工程资助项目;江苏省高校“青蓝工程”资助项目

Comparative Study on Autophagy of Standard Strains and Drug-resistant Strains in Candida Albicans

Received:2020-04-23 Revised:2020-05-26 Online:2020-08-29 Published:2020-08-28
Contact: Kai-Rui SUN E-mail:1627080053@qq.com

摘要/Abstract

摘要： 目的：比较白色念珠菌浮游状态耐药株与标准株自噬的差异，初步探讨自噬在白色念珠菌耐药中的作用。方法：RT-qPCR比较白色念珠菌耐药株与标准株自噬相关基因表达，ALP活性检测比较两组自噬水平，透射电镜观察耐药株与标准株的自噬现象。结果：RT-qPCR显示白色念珠菌耐药株与标准株自噬相关基因表达存在明显差异；ALP活性检测表明耐药株自噬活性较标准株高；透射电镜观察到耐药株中颗粒状的自噬小体。结论：白色念珠菌浮游状态耐药株与标准株自噬存在差异，提示耐药性的产生可能存在自噬的调节。

关键词: 白色念珠菌, 自噬, 浮游状态, 耐药株

Abstract: Objective To compare the differences of autophagy between drug-resistant strains and standard strains of planktonic Candida albicans, and to explore the role of autophagy in drug resistance in Candida albicans. Methods RT-qPCR was used to detect the expression of autophagy-related genes between the drug-resistant strains and the standard strains. Alkaline phosphatase (ALP) activity detection was performed to evaluate the level of autophagy between the two strains. The autophagosomes of the strains was observed by transmission electron microscopy (TEM). Results RT-qPCR results showed that the expression of autophagy-related genes was significantly different between the drug-resistant strains and the standard strains. ALP activity showed that compared with standard strains, the drug-resistant strains increased significantly. Autophagosomes were observed in drug-resistant strains under the transmission electron microscopy. Conclusion: The autophagy of the drug-resistant strains of Candida albicans was different from that of the standard strains and the emergence of drug resistance in Candida albicans may be regulated by autophagy.

Key words: Candida albicans, Autophagy, Planktonic, Drug resistance

中图分类号:

R781.5

孙凯瑞姜刘鎏周鹏顾静怡徐瑞魏昕. 白色念珠菌标准株及耐药株自噬的比较研究[J]. 口腔医学, 2020, 40(8): 677-681.

参考文献 32

[1]	Smeekens SP, RK Malireddi, TS Plantinga, et al. Autophagy is redundant for the host defense against systemic Candida albicans infections[J]. European Journal of Clinical Microbiology & Infectious Diseases, 2013, 33(5):711-722
[2]	Tan YQ, J Zhang, G Zhou.Autophagy and its implication in human oral diseases[J]. Autophagy, 2017, 13(2):225-236
[3]	Paul S, I Kannan, K Mohanram. Extensive ERG11 mutations associated with fluconazole-resistant Candida albicans isolated from HIV-infected patients[J]. Current Medical Mycology, 2019, 5(3):1-6
[4]	Shapiro RS, N Robbins, LE Cowen. Regulatory circuitry governing fungal development,drug resistance,and disease[J]. Microbiol Mol Biol Rev, 2011, 75(2):213-67
[5]	Peman J, E Canton, A Espinel-Ingroff. Antifungal drug resistance mechanisms[J]. Expert Rev Anti Infect Ther, 2009, 7(4):453-60
[6]	Sanguinetti M, B Posteraro, C Lass-Florl. Antifungal drug resistance among Candida species: mechanisms and clinical impact[J]. Mycoses, 2015, 58(2):2-13
[7]	Robbins N, T Caplan, LE Cowen. Molecular Evolution of Antifungal Drug Resistance[J]. Annu Rev Microbiol, 2017, 8(71):753-775
[8]	Alizadeh F, A Khodavandi, S Zalakian. Quantitation of ergosterol content and gene expression profile of ERG11 gene in fluconazole-resistant Candida albicans[J]. Curr Med Mycol, 2017, 3(1):13-19
[9]	Mohammadi R, H Zarrinfar, A Sardari. Detection of ERG11 point mutations in Iranian fluconazoleresistant Candida albicans isolates[J]. Current Medical Mycology, 2019, 5(1):7-14
[10]	Kanayama M, ML Shinohara. Roles of Autophagy and Autophagy-Related Proteins in Antifungal Immunity[J]. Frontiers in Immunology, 2016, 18(7):47-47
[11]	Palmer GE. Autophagy in the Invading Pathogen[J]. Autophagy, 2014, 3(3):251-253
[12]	Hale AN, DJ Ledbetter, TR Gawriluk, et al. Autophagy: regulation and role in development[J]. Autophagy, 2013, 9(7):951-72
[13]	Levine B, DJ Klionsky. Development by self-digestion: molecular mechanisms and biological functions of autophagy[J]. Dev Cell, 2004, 6(4):463-77
[14]	Li YJ, YH Lei, N Yao, et al. Autophagy and multidrug resistance in cancer[J]. Chin J Cancer, 2017, 36(1):52-52
[15]	Liu PF, KL Tsai, CJ Hsu, et al. Drug Repurposing Screening Identifies Tioconazole as an ATG4 Inhibitor that Suppresses Autophagy and Sensitizes Cancer Cells to Chemotherapy[J]. Theranostics, 2018, 8(3):830-845
[16]	Sarparanta J, M García-Macia, R Singh. Autophagy and Mitochondria in Obesity and Type 2 Diabetes[J]. Curr Diabetes Rev, 2017, 13(4):352-369
[17]	Rothe K, V Porter, X Jiang. Current Outlook on Autophagy in Human Leukemia: Foe in Cancer Stem Cells and Drug Resistance, Friend in New Therapeutic Interventions[J]. Int J Mol Sci, 2019, 20(3):461-461
[18]	Levy JM, JC Thompson, AM Griesinger, et al. Autophagy inhibition improves chemosensitivity in BRAF(V600E) brain tumors[J]. Cancer Discov, 2014, 4(7):773-80
[19]	Yu Q, C Jia, Y Dong, et al. Candida albicans autophagy, no longer a bystander: Its role in tolerance to ER stress-related antifungal drugs [J]. Fungal Genet Biol, 2015, Aug(81):238-249
[20]	虞丽华，马鸣，陈晓君，等. 白色念珠菌人工诱导耐药模型的建立及鉴定方法的研究[J]. 口腔生物医学, 2011, 2(2):75-77
[21]	张琰, 马鸣, 虞丽华, 等. 改良热酸酚法提取白色念珠菌早期生物膜总的研究[J]. 口腔生物医学, 2010, 001(002):65-67
[22]	Wen X, D Gatica, Z Yin, et al. The transcription factor Spt4-Spt5 complex regulates the expression of ATG8 and ATG41[J]. Autophagy, 2019, Sep(8):1-14
[23]	Rane HS, SM Bernardo, SR Hayek, et al. The contribution of Candida albicans vacuolar ATPase subunit V(1)B,encoded by VMA2,to stress response,autophagy,and virulence is independent of environmental pH[J]. Eukaryot Cell, 2014, 13(9):1207-21
[24]	Dong Y, Q Yu, Y Chen, et al. The Ccz1 mediates the autophagic clearance of damaged mitochondria in response to oxidative stress in Candida albicans[J]. The International Journal of Biochemistry & Cell Biology, 2015, Dec(69):41-51
[25]	Wei H, J-L Guan. Blocking tumor growth by targeting autophagy and SQSTM1 in vivo[J]. Autophagy, 2015, 11(5):854-855
[26]	Noda T, DJ Klionsky. The quantitative Pho8Delta60 assay of nonspecific autophagy[J]. Methods Enzymol, 2008, 451(451):33-42
[27]	Kainz K, J Tadic, A Zimmermann, et al. Methods to Assess Autophagy and Chronological Aging in Yeast[J]. Methods Enzymol, 2017, 588(588):367-394
[28]	Nothwehr SF, NJ Bryant, TH Stevens. The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins[J]. Mol Cell Biol, 1996, 16(6):2700-7
[29]	Huang J, JH Brumell. Bacteria-autophagy interplay: a battle for survival[J]. Nat Rev Microbiol, 2014, 12(2):101-14
[30]	Torggler R, Papinski D, Kraft C. Assays to Monitor Autophagy in Saccharomyces cerevisiae[J]. Cells, 2017, 6(23):1-24
[31]	Klionsky DJ, AM Cuervo, PO Seglen. Methods for monitoring autophagy from yeast to human[J]. Autophagy, 2007, 3(3):181-206
[32]	Klionsky DJ, K Abdelmohsen, A Abe, et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)[J]. Autophagy, 2016, 12(1):1-222

白色念珠菌标准株及耐药株自噬的比较研究

Comparative Study on Autophagy of Standard Strains and Drug-resistant Strains in Candida Albicans

可视化

摘要/Abstract

引用本文

使用本文

参考文献 32

相关文章 11

编辑推荐

Metrics

本文评价

[1]	陆嘉敏闫明李泽汉吴潇于金华. iRoot BP Plus对骨髓间充质干细胞成牙/成骨分化及自噬的影响[J]. , 2019, 39(10): 873-879.
[2]	王维倩黄震李靖敏丁子清. HEMA促进人牙源性间充质细胞自噬水平的作用研究[J]. , 2018, 38(12): 1084-1088.
[3]	王雅雯陈灼庚王彬娉程亚楠王秋子徐普. 缺氧下激活成骨细胞自噬对细胞增殖的影响[J]. , 2018, 38(10): 876-880.
[4]	张敏徐艳. 自噬在伴糖尿病牙周炎中的作用[J]. , 2017, 37(12): 1128-1131.
[5]	王超炜周益王慧明. 骨相关细胞的自噬及其在口腔种植中的作用[J]. , 2016, 36(5): 458-461.
[6]	王威栋李璐林丹萍夏鑫钰徐玲玲徐艳孙颖. 糖基化终产物对人牙周膜细胞自噬的影响[J]. , 2014, 34(8): 561-565.
[7]	裘黎路. 根管白色念珠菌感染1例[J]. , 2011, 31(03): 180-.
[8]	富丹丽,刘丽,章燕珍,梁伯贵,吕银祥. 白色念珠菌对钛合金及钴铬合金腐蚀影响的研究[J]. , 2009, 29(2): 84-85.
[9]	邹芳;刘卫红;陈俊根;贡艳宏. 不同赋形剂氢氧化钙抑制白色念珠菌的药敏试验[J]. , 2006, 26(1): 58-59.
[10]	杨筱荣. 口干症204例临床分析[J]. , 2003, 23(5): 298-299.
[11]	孙志英;陈仪. 伴糜烂的扁平苔藓与白色念珠菌关系的初步探讨[J]. , 1991, 11(2): 62-63.