微球在牙体组织再生领域应用的研究进展

doi:10.13591/j.cnki.kqyx.2022.04.016

摘要/Abstract

摘要： 随着组织工程技术和再生学科的发展,失去的牙体组织再生成为可能。微球作为组织工程中常用的载体形式,适用于小且形状不规则的牙体组织再生,同时还可利用微球的靶向性和缓释控释来运输各种生长因子、药物等到达指定区域。本文就微球在牙体组织再生领域应用的研究现状进行综述。

关键词: 微球, 牙体组织再生, 组织工程

Abstract: With the development of tissue engineering and regeneration, it is possible to regenerate the lost tooth tissue. As a commonly used carrier in tissue engineering, microspheres are suitable for small and irregular dental tissue regeneration. Locally available microspheres have been widely used as injectable cell carriers for tissue repair. At the same time, the targeting and sustained release of microspheres can be used to transport a variety of growth factors and drugs to the designated area. This paper reviews current research on application of microspheres in dental tissue regeneration.

Key words: microsphere, dental tissue regeneration, tissue engineering

中图分类号:

R780.2

张祎, 赵彬, 王璐, 姚蔚, 郝凤翔, 杨怡天. 微球在牙体组织再生领域应用的研究进展[J]. 口腔医学, 2022, 42(4): 362-367.

ZHANG Yi, ZHAO Bin, WANG Lu, YAO Wei, HAO Fengxiang, YANG Yitian. Progress of research on the application of microspheres in dental tissue regeneration[J]. Stomatology, 2022, 42(4): 362-367.

参考文献

[1] GBD Oral Disorders Collaborators, Bernabe E, Marcenes W, et al. Global, regional, and national levels and trends in burden of oral conditions from 1990 to 2017:A systematic analysis for the global burden of disease 2017 study[J]. J Dent Res, 2020, 99(4):362-373.
[2] Delaviz Y, Finer Y, Santerre JP. Biodegradation of resin composites and adhesives by oral bacteria and saliva:A rationale for new material designs that consider the clinical environment and treatment challenges[J]. Dent Mater, 2014, 30(1):16-32.
[3] Munari LS, Bowles WR, Fok ASL. Relationship between canal enlargement and fracture load of root dentin sections[J]. Dent Mater, 2019, 35(5):818-824.
[4] Angelova Volponi A, Zaugg LK, Neves V, et al. Tooth repair and regeneration[J]. Curr Oral Health Rep, 2018, 5(4):295-303.
[5] Hossain KMZ, Patel U, Ahmed I. Development of microspheres for biomedical applications:A review[J]. Prog Biomater, 2015, 4(1):1-19.
[6] Bu NU, Lee HS, Lee BN, et al. In vitro characterization of dental pulp stem cells cultured in two microsphere-forming culture plates[J]. J Clin Med, 2020, 9(1):242.
[7] Sun M, Liu A, Yang XF, et al. 3D cell culture—Can it be as popular as 2D cell culture?[J]. Adv Nanobiomed Res, 2021, 1(5):2000066.
[8] Chan YH, Lee YC, Hung CY, et al. Three-dimensional spheroid culture enhances multipotent differentiation and stemness capacities of human dental pulp-derived mesenchymal stem cells by modulating MAPK and NF-kB signaling pathways[J]. Stem Cell Rev Rep, 2021 (2021-04-24) [2021-08-19]. DOI:10.1007/s12015-021-10172-4.
[9] Wei Y, Wu YB, Wen K, et al. Recent research and development of local anesthetic-loaded microspheres[J]. J Mater Chem B, 2020, 8(30):6322-6332.
[10] 马士卿, 王洁, 高平, 等. 生物降解微球在骨组织再生中的生物学优势[J]. 中国组织工程研究, 2021, 25(34):5517-5522.
[11] Gupta V, Khan Y, Berkland CJ, et al. Microsphere-based scaffolds in regenerative engineering[J]. Annu Rev Biomed Eng, 2017, 19:135-161.
[12] Haugen HJ, Basu P, Sukul M, et al. Injectable biomaterials for dental tissue regeneration[J]. Int J Mol Sci, 2020, 21(10):E3442.
[13] Ibrahim MS, El-Wassefy NA, Farahat DS. Injectable gels for dental and craniofacial[J]. Applications Appl Biomed Eng Dent, 2020:359-375.
[14] Patel M, Jha A, Patel R. Potential application of PLGA microsphere for tissue engineering[J]. J Polym Res, 2021, 28(6):1-17.
[15] Simeonov M, Gussiyska A, Mironova J, et al. Novel hybrid chitosan/calcium phosphates microgels for remineralization of demineralized enamel: A model study[J]. Eur Polym J, 2019, 119:14-21.
[16] Pandya M, Diekwisch TGH. Enamel biomimetics-fiction or future of dentistry[J]. Int J Oral Sci, 2019, 11(1):8.
[17] Xiao Z, Que K, Wang H, et al. Rapid biomimetic remineralization of the demineralized enamel surface using nano-particles of amorphous calcium phosphate guided by chimaeric peptides[J]. Dent Mater, 2017, 33(11):1217-1228.
[18] Baranova J, Büchner D, Götz W, et al. Tooth formation:Are the hardest tissues of human body hard to regenerate?[J]. Int J Mol Sci, 2020, 21(11):4031.
[19] Padma Priya S, Higuchi A, Abu Fanas S, et al. Odontogenic epithelial stem cells:Hidden sources[J]. Lab Invest, 2015, 95(12):1344-1352.
[20] Ahmed GM, Abouauf EA, AbuBakr N, et al. Tissue engineering approaches for enamel, dentin, and pulp regeneration:An update[J]. Stem Cells Int, 2020, 2020:5734539.
[21] Hu X, Lee JW, Zheng X, et al. Efficient induction of functional ameloblasts from human keratinocyte stem cells[J]. Stem Cell Res Ther, 2018, 9(1):126.
[22] Sharma LA, Love RM. Scaffolds for regeneration of the pulp-dentine complex[M]//Handbook of Tissue Engineering Scaffolds:Volume One. Amsterdam:Elsevier, 2019:459-478.
[23] Kim SG. A cell-based approach to dental pulp regeneration using mesenchymal stem cells:A scoping review[J]. Int J Mol Sci, 2021, 22(9):4357.
[24] Eramo S, Natali A, Pinna R, et al. Dental pulp regeneration via cell homing[J]. Int Endod J, 2018, 51(4):405-419.
[25] Kim JY, Xin X, Moioli EK, et al. Regeneration of dental-pulp-like tissue by chemotaxis-induced cell homing[J]. Tissue Eng Part A, 2010, 16(10):3023-3031.
[26] Huang GT, Liu J, Zhu X, et al. Pulp/dentin regeneration:It should be complicated[J]. J Endod, 2020, 46(9s):S128-S134.
[27] Morsczeck C, Reichert TE. Dental stem cells in tooth regeneration and repair in the future[J]. Expert Opin Biol Ther, 2018, 18(2):187-196.
[28] Zein N, Harmouch E, Lutz JC, et al. Polymer-based instructive scaffolds for endodontic regeneration[J]. Materials (Basel), 2019, 12(15):E2347.
[29] 管东梅, 盛雪, 贾晓晓. 种子细胞在牙再生领域中的应用进展[J]. 全科口腔医学电子杂志, 2020, 7(1):28.
[30] Tsutsui TW. Dental pulp stem cells:Advances to applications[J]. Stem Cells Cloning, 2020, 13:33-42.
[31] Yang T, Zhang QY, Xie L, et al. hDPSC-laden GelMA microspheres fabricated using electrostatic microdroplet method for endodontic regeneration[J]. Mater Sci Eng C Mater Biol Appl, 2021, 121:111850.
[32] Zhang RT, Xie L, Wu H, et al. Alginate/laponite hydrogel microspheres co-encapsulating dental pulp stem cells and VEGF for endodontic regeneration[J]. Acta Biomater, 2020, 113:305-316.
[33] Garzón I, Martin-Piedra MA, Carriel V, et al. Bioactive injectable aggregates with nanofibrous microspheres and human dental pulp stem cells:A translational strategy in dental endodontics[J]. J Tissue Eng Regen Med, 2018, 12(1):204-216.
[34] Liu YS, Zhang T, Li M, et al. PLGA hybrid porous microspheres as human periodontal ligament stem cell delivery carriers for periodontal regeneration[J]. Chem Eng J, 2021, 420:129703.
[35] Kuang R, Zhang Z, Jin X, et al. Nanofibrous spongy microspheres enhance odontogenic differentiation of human dental pulp stem cells[J]. Adv Healthc Mater, 2015, 4(13):1993-2000.
[36] Wang W, Dang M, Zhang Z, et al. Dentin regeneration by stem cells of apical papilla on injectable nanofibrous microspheres and stimulated by controlled BMP-2 release[J]. Acta Biomater, 2016, 36:63-72.
[37] Liang C, Liao L, Tian WD. Stem cell-based dental pulp regeneration:Insights from signaling pathways[J]. Stem Cell Rev Rep, 2021, 17(4):1251-1263.
[38] Kishen A, Hussein H. Bioactive molecule carrier systems in endodontics[J]. Expert Opin Drug Deliv, 2020, 17(8):1093-1112.
[39] Li XW, Ma C, Xie XH, et al. Pulp regeneration in a full-length human tooth root using a hierarchical nanofibrous microsphere system[J]. Acta Biomater, 2016, 35:57-67.
[40] 李祥伟, 孙宏晨, 刘晓华. 载血管内皮细胞生长因子微球促进牙髓再生和血管形成的实验研究[J]. 中华口腔医学杂志, 2018, 53(1):42-48.
[41] Ortiz-Magdaleno M, Romo-Tobías AI, Romo-Ramírez F, et al. Behavior of mesenchymal stem cells obtained from dental tissues:A review of the literature[J]. Odovtos Int J Dent Sc, 2019, 21(1):31-40.
[42] Tran Hle B, Doan VN. Human dental pulp stem cells cultured onto dentin derived scaffold can regenerate dentin-like tissue in vivo[J]. Cell Tissue Bank, 2015, 16(4):559-568.
[43] Goldberg M. Scaffolds combined with stem cells have synergistic effect in regenerative dentistry[J]. J Clin Med Res, 2020, 1(3):1-11.
[44] Toledano M, Cabello I, Osorio E, et al. Zn-containing polymer nanogels promote cervical dentin remineralization[J]. Clin Oral Investig, 2019, 23(3):1197-1208.
[45] Zhang ML, Ni SL, Zhang X, et al. Dexamethasone-loaded hollow hydroxyapatite microsphere promotes odontogenic differentiation of human dental pulp cells in vitro[J]. Odontology, 2020, 108(2):222-230.
[46] Chang B, Ahuja N, Ma C, et al. Injectable scaffolds:Preparation and application in dental and craniofacial regeneration[J]. Mater Sci Eng R Rep, 2017, 111:1-26.
[47] Dave JR, Tomar GB. Dental tissue-derived mesenchymal stem cells:Applications in tissue engineering[J]. Crit Rev Biomed Eng, 2018, 46(5):429-468.
[48] Zhu LF, Dissanayaka WL, Zhang CF. Dental pulp stem cells overexpressing stromal-derived factor-1α and vascular endothelial growth factor in dental pulp regeneration[J]. Clin Oral Investig, 2019, 23(5):2497-2509.
[49] Zou HR, Wang GH, Song F, et al. Investigation of human dental pulp cells on a potential injectable poly(lactic-co-glycolic acid) microsphere scaffold[J]. J Endod, 2017, 43(5):745-750.
[50] 张璇, 李祥伟, 宋文龙, 等. 透明质酸微球的制备及细胞毒性的研究[J]. 实用口腔医学杂志, 2020, 36(6):954-958.
[51] Qian K, Li BQ, Zhu WH, et al. Preliminary evaluates of silica/β-TCP/PLGA microspheres for dentin regeneration in vivo[J]. Adv Appl Ceram, 2020, 119(5/6):357-363.
[52] 王冠华, 宋芳, 石旭东, 等. 人牙髓细胞在可注射PLGA微球支架上的粘附生长[J]. 牙体牙髓牙周病学杂志, 2017, 27(7):373-377,427.
[53] Ding CM, Chen ZX, Li JS. From molecules to macrostructures:Recent development of bioinspired hard tissue repair[J]. Biomater Sci, 2017, 5(8):1435-1449.
[54] 王珏,田子璐,刘艺萍,等.生物因子在牙骨质再生中的应用[J].中华口腔医学杂志,2019(8):568-572.
[55] Shuai Y, Ma Y, Guo T, et al. Dental stem cells and tooth regeneration[J]. Adv Exp Med Biol, 2018, 1107:41-52.
[56] Soudi A, Yazdanian M, Ranjbar R, et al. Role and application of stem cells in dental regeneration:A comprehensive overview[J]. Excli J, 2021, 20:454-489.
[57] Cho H, Tarafder S, Fogge M, et al. Periodontal ligament stem/progenitor cells with protein-releasing scaffolds for cementum formation and integration on dentin surface[J]. Connect Tissue Res, 2016, 57(6):488-495.