咬合改变和束缚应激中小鼠全脑神经激活的比较研究

doi:10.13591/j.cnki.kqyx.2022.06.001

口腔医学 ›› 2022, Vol. 42 ›› Issue (6): 481-486.doi: 10.13591/j.cnki.kqyx.2022.06.001

• 基础研究 • 下一篇

咬合改变和束缚应激中小鼠全脑神经激活的比较研究

徐明¹, 李娟¹, 刘伟才¹, 张琪²

1 同济大学口腔医学院·口腔医院修复教研室,上海牙组织修复与再生工程技术研究中心,上海(200072);
2 华东师范大学心理学院,上海市脑功能基因组学重点实验室,上海(200062)

修回日期:2022-03-08 出版日期:2022-06-28 发布日期:2022-07-21
通讯作者: 刘伟才 E-mail:vogi@163.com
基金资助:
国家自然科学基金青年基金(31800882);上海市自然科学基金项目面上项目(20ZR1463000);上海市自然科学基金启明星项目(19QA1407600)

Comparison of the activation of whole-brain neurons between occlusal change and restraint

XU Ming, LI Juan, LIU Weicai, ZHANG Qi

Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China

Revised:2022-03-08 Online:2022-06-28 Published:2022-07-21

摘要/Abstract

摘要： 目的观察咬合改变导致小鼠焦虑情绪后全脑神经元激活情况,并与束缚应激中全脑神经元激活情况进行比较,以探索咬合改变致小鼠焦虑情绪的神经机制。方法选取成年C57BL/6J雄性小鼠随机进行实验,咬合升高后第7、8天分别进行旷场测试、高架十字迷宫测试检测动物焦虑样行为。免疫荧光染色观察小鼠在咬合升高、束缚刺激后2 h全脑cfos蛋白表达情况。结果咬合升高后1周小鼠焦虑样行为显著增加(P＜0.05),咬合升高或束缚刺激2 h后全脑部分神经元激活,其中屏状核、伏隔核、外侧隔核、下丘脑室旁核、孤束核中表达cfos蛋白的神经元数目在两种刺激中均显著增加(P＜0.05)。结论咬合改变与束缚刺激后小鼠全脑激活神经元存在差异,但在屏状核、伏隔核、外侧隔核、下丘脑室旁核、孤束核中共同激活。

关键词: 咬合改变, 焦虑, 束缚, 小鼠

Abstract: Objective To observe the activation of neurons in the whole brain caused by occlusal changes in mice, and compare it with that in restraint stress in order to explore the neural mechanism of anxiety induced by occlusal changes in mice. Methods Adult C57BL/6J male mice were randomly selected for the experiment. On the 7th and 8th days after occlusal elevation, behavior test were performed after the surgery by analyzing movement in the open field and elevated plusmaze. The expression of cfos protein in the whole brain was detected by immunofluorescence in 2 hours after the surgery and the acute restraint stimuli. Results It was found that anxiety-like behaviors in OVR group increased significantly a week after surgery (P＜0.05), and the number of cfos-positive neurons in claustrum, nucleus accumbens, lateral septal nucleus, paraventricular hypothalamic nucleus and the nucleus of the solitary tract increased significantly in both stimuli (P＜0.05). Conclusion There are differences in the activation of neurons in the whole brain of mice after occlusal change or restraint, but they are co-activated in the claustrum, nucleus accumbens, lateral septal nucleus, paraventricular hypothalamic nucleus and the nucleus of the solitary tract.

Key words: occlusal change, anxiety, restraint, mice

中图分类号:

R780.1

徐明, 李娟, 刘伟才, 张琪. 咬合改变和束缚应激中小鼠全脑神经激活的比较研究[J]. 口腔医学, 2022, 42(6): 481-486.

XU Ming, LI Juan, LIU Weicai, ZHANG Qi. Comparison of the activation of whole-brain neurons between occlusal change and restraint[J]. Stomatology, 2022, 42(6): 481-486.

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咬合改变和束缚应激中小鼠全脑神经激活的比较研究

Comparison of the activation of whole-brain neurons between occlusal change and restraint

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