FAM20C在小鼠下颌髁突发育过程中的时空表达

doi:10.13591/j.cnki.kqyx.2022.07.005

口腔医学 ›› 2022, Vol. 42 ›› Issue (7): 600-603.doi: 10.13591/j.cnki.kqyx.2022.07.005

FAM20C在小鼠下颌髁突发育过程中的时空表达

祁皓¹, 马肃^1,2, 袁朝阳¹, 吴国栋³, 刘培红^1,2

1 哈尔滨医科大学附属第一医院·哈尔滨医科大学口腔医学院牙周科,黑龙江哈尔滨(150001);
2 哈尔滨医科大学附属第一医院龙江学者实验室,黑龙江哈尔滨(150001);
3 黑龙江省心血管病声光电磁诊疗重点实验室,黑龙江哈尔滨(150001)

修回日期:2022-02-22 出版日期:2022-07-28 发布日期:2022-07-22
通讯作者: 刘培红 Tel:(0451)85553435 E-mail:liupeihong76@163.com
基金资助:
黑龙江省自然科学基金面上项目(H2017030);哈尔滨医科大学附属第一医院科研基金(2016B020)

Spatial and temporal expression of FAM20C in mandibular condyle development of mice

QI Hao, MA Su, YUAN Zhaoyang, WU Guodong, LIU Peihong

Department of Periodontics, The First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University, Harbin 150001, China

Revised:2022-02-22 Online:2022-07-28 Published:2022-07-22

摘要/Abstract

摘要： 目的观察小鼠髁突发育过程的不同阶段,FAM20C在髁突软骨中的时空表达特点,试探究其在小鼠髁突发育过程中的可能作用机制。方法苏木精-伊红(HE)染色和改良番红O-固绿染色观察胚胎17.5 d和出生后0、7、21 d 4组小鼠髁突软骨及软骨下骨的形态学变化。免疫组化染色观察相应时间点FAM20C在小鼠髁突软骨组织中的定位及表达。测量FAM20C阳性表达的平均光密度值,并进行单因素方差分析。结果 HE染色和改良番红O-固绿染色结果表明:随着软骨内骨化的进展,髁突软骨细胞层长度减少,软骨下骨体积增加,下颌髁突体积增大;免疫组化结果表明:4组小鼠髁突软骨组织中均有FAM20C阳性表达,FAM20C主要表达于髁突软骨增殖软骨细胞层和前肥大软骨细胞层,少量表达于肥大软骨细胞层及软骨下骨层,随着髁突发育,FAM20C表达逐渐减少。统计学结果显示,各时间点FAM20C阳性表达差异有统计学意义(P<0.01)。结论 FAM20C参与小鼠下颌髁突发育,可能通过调节髁突软骨细胞的增殖和分化在髁突形成中发挥重要作用。

关键词: FAM20C, 髁突软骨, 发育, 时空表达, 小鼠

Abstract: Objective To observe the spatial and temporal expression of FAM20C in condylar cartilage at different stages of condylar development in mice and explore its possible mechanism. Methods HE staining and modified safranin O-fast green staining were used to observe morphological changes of condylar cartilage and subchondral bone in 17.5 days of embryo and 0, 7, and 21 days after birth. Immunohistochemical staining was used to observe the localization and expression of FAM20C in mouse condylar cartilage tissues at corresponding time points. The mean optical density values of FAM20C positive expression were measured and subjected to one-way ANOVA. Results Results of HE staining and modified safranin O-fast green staining showed that with the progression of endochondral ossification, the length of condylar chondrocyte layer decreased; the volume of subchondral bone increased, and the volume of mandibular condyle increased. The immunohistochemical results showed that FAM20C was positively expressed in condylar cartilage tissue of the four groups of mice. FAM20C was mainly expressed in the proliferating chondrocyte layer, and pre-hypertrophic chondrocyte layer of condylar cartilage and a small amount was expressed in the hypertrophic chondrocyte layer and subchondral bone layer. With the development of condyle, the expression of FAM20C gradually decreased. Results of statistical analysis showed that there was a significant difference in FAM20C positive expression at each time point (P<0.01). Conclusion FAM20C is involved in mandibular condyle development in mice and may play an important role in condyle formation by regulating the proliferation and differentiation of condylar chondrocytes.

Key words: FAM20C, condylar cartilage, development, temporal and spatial expression, mice

中图分类号:

R321.53

祁皓, 马肃, 袁朝阳, 吴国栋, 刘培红. FAM20C在小鼠下颌髁突发育过程中的时空表达[J]. 口腔医学, 2022, 42(7): 600-603.

QI Hao, MA Su, YUAN Zhaoyang, WU Guodong, LIU Peihong. Spatial and temporal expression of FAM20C in mandibular condyle development of mice[J]. Stomatology, 2022, 42(7): 600-603.

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FAM20C在小鼠下颌髁突发育过程中的时空表达

Spatial and temporal expression of FAM20C in mandibular condyle development of mice

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