杨梅素对脱矿牙本质再矿化及树脂-牙本质粘接效果的影响

doi:10.13591/j.cnki.kqyx.2022.10.001

口腔医学 ›› 2022, Vol. 42 ›› Issue (10): 865-868.doi: 10.13591/j.cnki.kqyx.2022.10.001

• 基础研究 • 下一篇

杨梅素对脱矿牙本质再矿化及树脂-牙本质粘接效果的影响

王贝贝¹, 韩菲¹, 袁晓君², 陈晨², 谢海峰¹

1 南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院修复科,江苏南京(210029);
2 南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院牙体牙髓科,江苏南京(210029)

修回日期:2022-07-07 出版日期:2022-10-28 发布日期:2022-10-28
通讯作者: 谢海峰 Tel:(025)69593081, E-mail:xhf-1980@126.com
基金资助:
国家自然科学基金(81970927);江苏省卫健委医学科研项目(M2020066);江苏高校优势学科建设工程(2018-87)

Effects of myricetin on dentin remineralization and bonding strength

WANG Beibei, HAN Fei, YUAN Xiaojun, CHEN Chen, XIE Haifeng

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University; Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China

Revised:2022-07-07 Online:2022-10-28 Published:2022-10-28

摘要/Abstract

摘要： 目的评价杨梅素(myricetin,MYR)对脱矿牙本质基质的再矿化效果及其对牙本质粘接强度的影响。方法切取中层牙本质,使用600目碳化硅砂纸湿抛光模拟玷污层,35%磷酸酸蚀后分别给予蒸馏水浸泡、直接再矿化处理2 d和MYR预处理30 min后再矿化处理2 d。采用扫描电镜(scanning electron microscope,SEM)观察样本表面形态,X射线衍射(X-ray diffraction,XRD)和衰减全反射傅里叶变换红外光谱(attenuated total reflection Fourier transform infrared spectroscopy,ATR-FTIR)分析表面物质,微拉伸强度(micro tensile strength,μTBS)和纳米渗漏实验观察粘接效果。结果 SEM发现再矿化处理的各组牙本质片均可以形成晶体,其中经MYR预处理后再矿化的样本形成的晶体最为明显,新形成的晶体经XRD和ATR-FTIR分析为羟基磷灰石。μTBS和纳米渗漏实验发现MYR预处理后的牙本质试件再矿化可明显增加粘接强度,减少纳米渗漏。结论 MYR预处理可以促进脱矿牙本质的再矿化过程并改善树脂-牙本质粘接强度。

关键词: 牙本质, 杨梅素, 再矿化, 羟基磷灰石, 粘接

Abstract: Objective To evaluate the remineralization effect of myricetin (MYR) on demineralized dentin matrix and its effect on dentin bonding strength. Methods The middle dentin slices were obtained and then were wet polished with 600 mesh silicon carbides and paper to simulate the smear layer. The dentin slices were etched with 35% phosphoric acid and divided into three groups, which were respectively immersed in distilled water, directly remineralized for 2 d and pretreated with MYR for 30 min and then remineralized for 2 d. Surface morphology of the dentin slices was observed with scanning electron microscope(SEM), and surface materials were analyzed with X-ray diffraction(XRD)and attenuated total reflection Fourier transform infrared spectroscopy(ATR-FTIR). Micro tensile strength (μTBS)and nano-leakage experiments were used to observe the bonding effect. Results SEM showed that the dentin after remineralization could form crystals, while crystals formed by remineralized dentin after MYR pretreatment were the most obvious. XRD and ATR-FTIR analysis revealed that the newly formed crystal was hydroxyapatite(HA). μTBS and nanoleakage experiments showed that remineralization after MYR pretreatment could significantly increase bond strength and reduce nanoleakage. Conclusion MYR pretreatment can promote the remineralization process of demineralized dentin and improve the resin/dentin bonding strength.

Key words: dentin, myricetin, remineralization, hydroxyapatite, bonding

中图分类号:

R783.1

王贝贝, 韩菲, 袁晓君, 陈晨, 谢海峰. 杨梅素对脱矿牙本质再矿化及树脂-牙本质粘接效果的影响[J]. 口腔医学, 2022, 42(10): 865-868.

WANG Beibei, HAN Fei, YUAN Xiaojun, CHEN Chen, XIE Haifeng. Effects of myricetin on dentin remineralization and bonding strength[J]. Stomatology, 2022, 42(10): 865-868.

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杨梅素对脱矿牙本质再矿化及树脂-牙本质粘接效果的影响

Effects of myricetin on dentin remineralization and bonding strength

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