Mg-2Zn-0.5Ca合金/矿化胶原复合物修复兔下颌骨缺损的实验研究

doi:10.13591/j.cnki.kqyx.2022.12.003

摘要/Abstract

摘要： 目的研究用于修复兔下颌骨缺损的Mg-2Zn-0.5Ca合金/矿化胶原复合物金属-骨界面及成骨状况。方法取40只日本大耳兔,随机分为4组:Mg-2Zn-0.5Ca合金/矿化胶原组(A组);矿化胶原组(B组);Mg-2Zn-0.5Ca合金组(C组);空白对照组(D组)。于兔的下颌骨缺牙区域制备长10 mm、宽7 mm、厚2 mm的块状骨缺损,并保留舌侧骨板; A组植入Mg-2Zn-0.5Ca合金/矿化胶原复合物;B组植入矿化胶原;C组植入Mg-2Zn-0.5Ca合金材料;D组不植入材料。分别在预定的时间点对材料和大耳兔进行肉眼观察、血清学检测、X线片、扫描电镜(SEM)、组织学观察。结果 A、B、C、D组血清中镁离子浓度在植入材料前后无显著性差异;C组术后尿中镁离子浓度明显高于术前。术后12周,A组和B组可见有新形成的骨组织,C组可见有软骨样组织,没有发现明显的骨小梁结构。X线显示:A 组和C组材料周边有不同程度的降解;C组材料比A组材料周围有更明显的阴影。SEM显示:A组材料表面变粗糙,降解不明显,有骨组织附着;C组材料出现明显层状崩解,表面没有组织附着。组织学检查显示A组、B组材料周围有新骨小梁结构和成骨细胞形成,C组材料周围有软骨样组织。结论 Mg-2Zn-0.5Ca合金/矿化胶原不会造成血清中镁含量增高,可以降低其降解速率,具有良好的成骨性能。

关键词: Mg-2Zn-0.5Ca合金/矿化胶原复合物, 生物相容性, 成骨状况, 兔

Abstract: Objective To evaluate metal-bone interface and bone formation of Mg-2Zn-0.5Ca alloy/mineralized collagen in bone defect repair. Methods Forty Japanese rabbits were randomly divided into four groups: Mg-2Zn-0.5Ca alloy/mineralized collagen (group A), mineralized collagen (group B), Mg-2Zn-0.5Ca alloy (group C) and blank control group (group D). The cheek side of rabbit mandible was drilled with 10 mm×7 mm×2 mm defect, similar to the defect of bone window. Mg-2Zn-0.5Ca alloy/mineralized collagen was implanted in group A; mineralized collagen was implanted in group B; Mg-2Zn-0.5Ca alloy was implanted in group C; no materials were implanted in group D. They were evaluated in biocompatibility and osteogenesis through visual inspection, serological examination, X-ray, SEM and histology at the pre-set time point. Results There was no significant difference in serum magnesium ion concentration of group A, B, C and D; in group C, the concentration of magnesium ion in urine after operation was significantly higher than that before operation. At 12 weeks after operation, new bone tissue was found in group A and group B; cartilage-like tissue was found in group C, and trabecular bone structure was not found. X-ray showed that there were different degrees of degradation around the materials in group A and group C; there was more obvious shadow around group C than group A. SEM showed that the surface of group A became rough and the degradation was not obvious, and there was bone tissue attached to the surface; materials in group C showed obvious layered disintegration, and there was no tissue attached. Histological examination showed that there was bone trabeculaes and osteoblasts around group A and group B, cartilage-like tissue around group C. Conclusion The Mg-2Zn-0.5Ca alloy/mineralized collagen does not increase the content of magnesium in serum and can reduce the degradation rate. Mg-2Zn-0.5Ca alloy/mineralized collagen has good osteogenic performance.

Key words: Mg-2Zn-0.5Ca alloy/mineralized collagen, biocompatibility, osteogenesis, rabbit

中图分类号:

R783.1

王树峰, 陈苗苗, 王程越. Mg-2Zn-0.5Ca合金/矿化胶原复合物修复兔下颌骨缺损的实验研究[J]. 口腔医学, 2022, 42(12): 1067-1072.

WANG Shufeng, CHEN Miaomiao, WANG Chengyue. Experimental study on repairing rabbit mandibular defect with Mg-2Zn-0.5Ca alloy/mineralized collagen[J]. Stomatology, 2022, 42(12): 1067-1072.

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