›› 2016, Vol. 36 ›› Issue (6): 503-507.

• Basic Research • Previous Articles     Next Articles

Effect of BioAggregate and MTA on osteoclast differentiation and function in vivo

  

  • Received:2015-09-14 Revised:2016-01-08 Online:2016-06-28 Published:2016-06-30

Abstract: Objective  To investigate the effect of BioAggregate on LPS-induced bone destruction in vivo, and to compare its performance with that of mineral trioxide aggregate. Methods Six-week-old C57/BL6 male mice were divided into the injection group containing 10 mg/kg LPS with or without the material extracts (n=6) and the vehicle-injection group with the culture medium alone (n=6). After LPS stimulation for 7 days, mice were sacrificed and calvarial bones were prepared for micro-computed tomography scanning, histologic analysis and double immunofluorescence staining. Results BioAggregate and MTA extracts markedly reduced osteoclast numbers and attenuated bone resorption in LPS-challenged mouse calvaria. The expression level of osteoclastogenic cathepsin K was also decreased by BioAggregate and MTA extracts. Conclusions BioAggregate and MTA showed comparable inhibitory effect on osteoclast differentiation and inflammatory bone resorption in vivo.

Key words: BioAggregate, MTA, osteoclast

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