基于iTRAQ技术的伴放线聚集杆菌细胞致死性扩张毒素诱导Jurkat细胞凋亡研究

Abstract

Abstract: Abstract: Objective To explore the key signaling molecules when cytolethal distending toxin (CDT), a kind of Aggregatibacter actinomycetemcomitans (A.a) induced apoptosis of T lymphocytes in vitro. Methods Jurkat cells were cultured and then divided into groups: the control group, the wild group (Jurkat cells were stimulated by wild A.a CDT), and the mutant group (Jurkat cells were stimulated by mutant A.a CDT). After human Jurkat cells were treated by A.a CDT for 24 hours, iTRAQ (Isobaric tags for relative and absolute quantitation) labeling technique was used to test differentially expressed apoptosis-relating proteins. Results 20 apoptosis-related differentially expressed proteins (fold change ratio>1.3) were identified, including 8 proteins (CASP8/CD3E/YY1/SH3BGRL3/P53/CASP3/UBE2I/ TNFRSF10B) which were upregulated and 12 proteins (F5/RPL18A/DAD1/MTCH2/HIST1H1E/CNBP/RBM25/SLC16A1/KDM2A/CD99/RAC2/TMX1) which were downregulated. Conclusion This study elucidates the signaling molecules when A.a CDT induced apoptosis of human Jurkat cells at the molecular level, and one possible signaling pathway was selected (UBE2I/P53/TNFRSF10B/CASP8/CASP3), providing thoughts for the future studies and the treatment of localized aggressive periodontitis.

Key words: Aggregatibacter actinomycetemcomitans, Cytolethal distending toxin, Jurkat cells, Apoptosis, iTRAQ labeling

CLC Number:

R780.2

References

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Study of Aggregatibacter actinomycetemcomitans cytolethal distending toxin on inducing apoptosis in human Jurkat cells by iTRAQ labeling technique

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