Abstract: Objective To make a preliminary evaluation of the adhesive properties and antibacterial properties of methyl methacrylate bound sixteen alkyl two-ammonium chloride (DMAE-CB) and amorphous calcium phosphate (NACP) binder. Methods DMAE-CB and NACP were added to the commercial dentin adhesive according to different proportions. According to the different proportions, the experiment was divided into 8 groups, the commercialization of binder as the control group. The dentin shear strength was tested by universal testing machine, and the bacteriostatic experiment was conducted, the antibacterial activity against Streptococus mutans of different groups after adhesion was studied. Results The shear strength of control group was （17.96±3.45）MPa, the experimental group were A：（19.25±2.80）MPa, B：（18.70±2.42）MPa, C：（18.47±2.73）MPa, D：（18.22±2.50）MPa，E：（17.29±4.54）MPa, F：（18.40±2.98）MPa, G：（18.13±4.39）MPa, through statistical analysis of P=0.993 (P>0.05) the shear strength of the experimental group and the control group the difference was statistically significant; the control group in the contact bacteriostatic test had antibacterial effect, the experimental group of antibacterial monomer addition 1% had significant antibacterial effects (P＜0.05), when the dosage is 3%, the antibacterial activity was further enhanced, but when the content is 4%, the antibacterial activity did not significantly increase. Conclusion After adding DMAE-CB and NACP, the adhesive has no influence on the bond strength and has good antibacterial properties.

Key words: antibacterial activity, dentin adhesive, shear bond strength, DMAE-CB, NACP