HEMA促进人牙源性间充质细胞自噬水平的作用研究

Abstract

Abstract: Objective　 To investigate the autophagy-induction effect of 2-Hydroxyethyl methacrylate(HEMA) on human dental mesenchymal cells (DMCs). Methods　DMCs were obtained from freshly extracted healthy premolars and third molars from patients (15–22 years old) with orthodontic and preventive teeth extraction. Cells of the 4th-6th generations were treated by different concentrations of HEMA for different periods. Cell viability was detected by CCK-8 assessment after treated with HEMA (0,2,4,6,8,10,12 mmol/L) for 12, 24, 48 h. Cell phenotype change was detected by Phalloidin staining. AO staining was used to observe the vesicular organelles in DMCs. Western blot assessment was applied to detected the protein expressions of Beclin1, Atg5 and LC3II. Results　The cell viability decreased with HEMA treatment in a dose- and time-dependent manner. The cell phenotype was changed by HEMA treatment. Increasing numbers of vesicular organelles were observed in the cytoplasm of HEMA-treated DMCs. The protein expressions of Beclin1, Atg5 and LC3II were elevated by HEMA treatment in DMCs in dose-dependent manner. Conclusion　HEMA greatly enhances the autophagy induction in a dose –dependent manner, which contributes to the cytotoxicity in DMCs. Its mechanism is associated with the increase of cell autophagy activation levels.

Key words: human dental mesenchymal cells, cytotoxicity, autophagy

CLC Number:

R329.28

References

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Effect study on HEMA promoting the autophagy processin human dental mesenchymal cells

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