长链非编码RNA MEG3对头颈鳞状细胞癌增殖和侵袭的影响

Abstract

Abstract: Abstract Objective: To investigate the effects of long non-coding RNA maternally expression gene 3 (MEG3) on proliferation and invasion of head and neck squamous cell carcinoma (HNSCC). Methods: Cultured the head and neck squamous cell carcinomacell line Cal27 in vitro，and they were divided into negative transfection group (MEG3 control ) and MEG3 Lentivirus overexpression transfection group (MEG3 overexpression) after the transfection of MEG3，real-time quantitative PCR(qRT－PCR) was used to detect MEG3 mRNA expression，CCK-8 assay was used to detect cell proliferation; transwell was used to detect cell migration; western blotting (WB) was used to detect the expressions of ZEB2，Vimentin and E-cadherin in cells．Results: In Cal27 cell， MEG3 mRNA expression was increased in the MEG3 overexpression group compared with the MEG3 control group (P<0.05).Compared with the control group, cell migration and invasion in the MEG3 overexpression group were decreased (P＜0.05)．Compared with control group，the expressions of ZEB2 and vimentin protein in MEG3 overexpression group were decreased，and the expression of E-cadherin protein was increased (P＜0.05)．Conclusion: The increase of MEG3 expression can inhibit the proliferation and invasion of head and neck squamous cell carcinomacells，the mechanism may be related to the inhibition of epithelial cell mesenchymal transition ( EMT)．

Key words: head and neck squamous cell carcinoma (HNSCC), maternally expression gene 3 (MEG3), proliferation, invasion, epithelial cell mesenchymal transition （EMT)

CLC Number:

R739.9

References

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Influences of long non-coding RNA MEG3 on the proliferation and invasion of HNSCC

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