Abstract: Objective　 To evaluate the differences in the cytokine release in vitro and the degradation rate in vivo of concentrated growth factor (CGF) membranes for standardized protocol of CGF membrane preparation with three designed compression methods: dry gauze, a compressor and a special syringe. Methods Microsurface textures and fibrin densities of the compressed CGF membranes were examined via scanning electron microscopy and transmission electron microscopy. Cytokine release (TGF-β1, VEGF, PDGF-AB) and the degradation rate were analyzed with one-way ANOVA and Kruskal-Wallis H test by conducting enzyme-linked immunosorbent assay, haematoxylin-eosin and Mallory phosphotungstic acid-haematoxylin staining. Results No significant difference in the release of main cytokines and the degradation rate was noted amongst the three compression methods, although the surface topographies and fibrin densities of the three-group membranes were quite different. The main cytokines in vitro exhibited steady controlled release until the 33rd day, and the release amount did not decrease evidently at different time points. In vivo, all the fibrin membranes were degraded completely within 5 weeks. Conclusions No significant difference was found in cytokine release and degradability amongst the CGF membranes with different compression methods. The results provided theoretical basis for standardized protocol of CGF membrane preparation.

Key words: concentrated growth factors, cytokine release, degradation