牙龈卟啉单胞菌对db/db小鼠骨髓来源巨噬细胞极化作用的初步探究

Abstract

Abstract: Objective To investigate the effects of porphyromonas gingivalis (Pg) on polarization of bone marrow-derived macrophages (BMDMs) in db/db mice. Methods Pg was used to stimulate BMDMs from wild type C57BL / 6 or db/db mice, the expression level of M1 and M2 gene markers was detected. The relative expression levels of Tnf-α, Il-12, Arg1, Ym1, Fizz1 and Mrc1 were detected by qRT-PCR. Results Compared with wild type mice, there was no statistically significant difference in the effect of Pg infection or not on BMDMs expressing Arg1, Fizz1 and Mrc1 in db/db mice under IL-4 induction; however, the expression of Ym1 in BMDMs of db/db mice was significantly decreased, while no significant difference was found in Tnf-α, and the expression of il-12 was markedly decreased. Conclusion The ability for Pg to switch from M2 to M1 was weakened in BMDMs of db/db mice.

Key words: Key words: periodontitis, porphyromonas gingivalis, type 2 diabetes mellitus, macrophage polarization

CLC Number:

R781.42

References 19

[1]	Shoelson S E, Lee J, Goldfine A B.Inflammation and insulin resistance[J].J Clin Invest, 2006, 116(7):1793-801
[2]	Hirosumi J, Tuncman G, Chang L, et al.A central role for JNK in obesity and insulin resistance[J].Nature, 2002, 420(6913):333-6
[3]	Olefsky J M, Glass C K.Macrophages, inflammation, and insulin resistance[J]. Annu Rev Physiol, 2010, 72: 219-46.[J].Annu Rev Physiol, 2010, 72:219-46
[4]	Chawla A, Nguyen K D, Goh Y P.Macrophage-mediated inflammation in metabolic disease[J].Nat Rev Immunol, 2011, 11(11):738-49
[5]	Mochin M T, Underwood K F, Cooper B, et al.Hyperglycemia and redox status regulate RUNX2 DNA-binding and an angiogenic phenotype in endothelial cells[J]. Microvasc Res, 2015, 97: 55-64.[J].Microvasc Res, 2015, 97:55-64
[6]	Gyurko R, Siqueira C C, Caldon N, et al.Chronic hyperglycemia predisposes to exaggerated inflammatory response and leukocyte dysfunction in Akita mice[J].J Immunol, 2006, 177(10):7250-6
[7]	Auyeung L, Wang P W, Lin R T, et al.Evaluation of periodontal status and effectiveness of non-surgical treatment in patients with type 2 diabetes mellitus in Taiwan for a 1-year period[J].J Periodontol, 2012, 83(5):621-8
[8]	Devanoorkar A, Dwarakanath C D, Gundanavar G, et al.Evaluation of serum resistin levels in periodontal health and disease and effects of non surgical periodontal therapy on its levels[J].Dis Markers, 2012, 32(5):289-94
[9]	Yu S, Ding L, Liang D, et al.Porphyromonas gingivalis inhibits M2 activation of macrophages by suppressing alpha-ketoglutarate production in mice[J].Mol Oral Microbiol, 2018, 33(5):388-395
[10]	Wu C, Liu C, Luo K, et al.Changes in Expression of the Membrane Receptors CD14,MHC-II,SR-A,and TLR4 in Tissue-Specific MonocytesMacrophages Following Porphyromonas gingivalis-LPS Stimulation[J].Inflammation, 2018, 41(2):418-431
[11]	Kamano Y, Watanabe J, Iida T, et al.Binding of PICK1 PDZ domain with calcineurin B regulates osteoclast differentiation[J].Biochem Biophys Res Commun, 2018, 496(1):83-88
[12]	Carley A N, Severson D L.What are the Biochemical Mechanisms Responsible for Enhanced Fatty Acid Utilization by Perfused Hearts from Type 2 Diabetic dbdb Mice?[J].Cardiovascular Drugs and Therapy, 2008, 22(2):83-89
[13]	Singh V P, Gurunathan C, Singh S, et al.Genetic deletion of Wdr13 improves the metabolic phenotype of Leprdbdbmice by modulating AP1 and PPARγ target genes[J].Diabetologia, 2015, 58(2):384-392
[14]	elechowska P, Agier J, Ró?alska S, et al.Leptin stimulates tissue rat mast cell pro-inflammatory activity and migratory response[J].Inflammation research : official journal of the European Histamine Research Society ... [et al., 2018, 67(9):789-799
[15]	吴静, 王华旻, 李晶.小鼠的实验室应用[J].生理科学进展, 2013, 44(1):12-18
[16]	Cucak H, Grunnet L G, Rosendahl A.Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarization[J].Journal of Leukocyte Biology, 2014, 95(1):149-160
[17]	Choi K M, Kashyap P C, Dutta N, et al.CD206-positive M2 macrophages that express heme oxygenase-1 protect against diabetic gastroparesis in mice[J].Gastroenterology, 2010, 138(7):2399-2409
[18]	Naylor C, Petri W A.Leptin Regulation of Immune Responses[J].Trends in Molecular Medicine, 2016, 22(2):88-98
[19]	Zhou Y, Yu X, Chen H, et al.Leptin Deficiency Shifts Mast Cells toward Anti-Inflammatory Actions and Protects Mice from Obesity and Diabetes by Polarizing M2 Macrophages[J].Cell metabolism, 2015, 22(6):1045-1058

A preliminary study of polarization of bone marrow-derived macrophages stimulated by porphyromonas gingivalis in db/db mice

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