透明质酸及其合成酶在炎症牙髓组织中的表达

Abstract

Abstract: Objective To detect the expression of the hyaluronic acid (HA) and hyaluronic acid synthases (HAS1, HAS2, HAS3) in the inflamed pulp tissue, and provide new information for their roles in the process of pulp inflammation.Methods Pulp tissues from the fully impacted mandibular third molars and the erupted third molars in a state of inflammation caused by deep caries in healthy patients were collected. The expression of HA, HAS1, HAS2, HAS3 and TNFα in the collected dental pulp were detected by immunohistochemical staining and immunofluorescence staining. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect HAS1, HAS2, HAS3 and the related inflammatory cytokines IL-6, IL-8, TNFα in the collected samples. Results The IHC and IF results showed that in the inflamed pulp tissue, the expression of HA, HAS2 and HAS3, which were co-stained with TNFα, were stronger compared with in healthy pulp tissue. The expression of HAS2 in the inflamed pulp tissue was much stronger than that of HAS3, and the expression of HAS1 was weak in healthy and inflamed pulp tissue. The results of qRT-PCR indicated that in the inflamed pulp, the expression levels of IL-6, IL-8, TNFα, HAS1, HAS2 and HAS3 (PIL-6, IL-8, TNFα, HAS1, HAS2, HAS3<0.01) were significantly increased compared with in the normal pulp. Conclusion The expression of HA, HAS2 and HAS3 were significantly up-regulated in the dental pulp tissue under inflammation. This suggests that HA may be involved in regulating the inflammatory state of dental pulp tissue.

Key words: hyaluronan, hyaluronan synthase, pulp, inflammation

CLC Number:

R781.31

Wei-Ting Chen Beizhan /Jiang. Expression of hyaluronic acid and hyaluronic acid synthases in dental pulp tissue under inflammation[J]. , 2020, 40(7): 606-611.

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Expression of hyaluronic acid and hyaluronic acid synthases in dental pulp tissue under inflammation

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