大蒜素对口腔鳞状细胞癌治疗作用的研究

Abstract

Abstract: Objective To investigate the therapeutic effect of Allicin on oral squamous cell carcinoma by in vitro studies, and to analyze the possible mechanism by network pharmacology. Methods The effects of Allicin on the proliferation, migration and invasion of SCC-15 cells were detected by CCK-8 method, scratch test and Transwell test. According to the 2D and 3D structure of Allicin, the potential target genes of Allicin were predicted. The possible pathogenic genes of OSCC were obtained by GEO chip, and the protein interaction network was constructed. The KEGG pathway and GO function in the enriched network were analyzed by bioinformatics method. Results When the concentration of Allicin was over 200 μmol/L, the proliferation of SCC-15 cells was significantly inhibited, and the inhibition increased with the increase of time and concentration (P<0.01). Among 104 potential target genes of Allicin, 12 genes intersected with oral squamous cell carcinoma target genes. KEGG analysis revealed that the intersection was related to cell cycle, p53 signaling pathway and platinum resistance. Go analysis indicated that intersection had multiple effects on biological process, molecular function and cell components of OSCC cells. Conclusion Allicin may play a therapeutic role in oral squamous cell carcinoma cells by affecting multiple signal pathways through multiple targets.

Key words: Allicin, OSCC, Migration, Invasion, Network pharmacology

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References 21

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Therapeutic effect of Allicin on oral squamous cell carcinoma

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