›› 2020, Vol. 40 ›› Issue (9): 796-799.

• Basic Research • Previous Articles     Next Articles

Effects of two sterilization methods on sterilization efficacy and bonding strength of extracted human teeth

  

  • Received:2019-09-24 Revised:2020-01-03 Online:2020-09-28 Published:2020-10-07
  • Contact: Lu Shi E-mail:lsrq@zzu.edu.cn

Abstract: Objective To investigate the effectiveness of steam autoclave and hydrogen peroxide plasma sterilization technology (HPPST) on extracted human teeth and the influence of sterilization methods on bond strength of dentin adhesive system. Methods 40 extracted human premolars were collected and divided evenly into two groups randomizely: steam autoclave group and HPPST group. Another 10 freshly extracted teeth without sterilization were used as positive control for the bond strength test. After treated by the two sterilization methods respectively, the effectiveness of sterilizations were determined by agar plate culture. Composite resins were bonded to the dentins by Clearfil SE Bond. Then the bond strength was detected by shear force experiment. Results After treated by the two sterilization methods respectively, all the samples were effectively sterilized determined by agar plate culture. Compared with the freshly extracted teeth (64.37±4.88MPa), the sterilized teeth (47.66±1.22MPa) had significantly lower bond strength with the HPPST group (36.88±2.61MPa) being the lowest. Conclusion Both steam autoclave and hydrogen peroxide plasma sterilization technology were suitable to sterilize extracted human teeth but resulted in significantly lower bond strength.

Key words: extracted human tooth, hydrogen peroxide plasma sterilization technology, steam autoclave, bond strength

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