肉桂醛对口腔鳞状细胞癌细胞CAL27增殖和迁移的影响

Abstract

Abstract: Objective　 To study the effect of different concentrations of cinnamaldehyde on the proliferation, migration, invasion, apoptosis and epithelial-mesenchymal transition of oral squamous cell carcinoma (OSCC) CAL27 cells in two dimensional conditions in vitro. Methods CCK8 test was performed to determine whether cinnamaldehyde can inhibit the proliferation of OSCC CAL27. Scratch test was conducted to determine whether cinnamaldehyde has an inhibitory effect on the migration of CAL27. Transwell test was used to test whether cinnamaldehyde can inhibit the invasion of CAL27. Flow cytometry was used to test whether cinnamaldehyde can promote the apoptosis of CAL27. Western blot was performed to determine whether cinnamaldehyde has an effect on the epithelial-mesenchymal transition of CAL27. Results The results of CCK8 showed that the experimental group treated with cinnamaldehyde had a time and drug concentration dependence effect on inhibiting the proliferation of CAL27. The results of scratch test and transwell test showed that the experimental group treated with cinnamaldehyde inhibited the cell migration ability and invasion ability in a dose-dependent manner. The results of flow cytometry showed that the experimental group treated with cinnamaldehyde promoted the apoptosis of CAL27 in a dose-dependent manner. The results of Western blot showed that the experimental group treated with cinnamaldehyde inhibited the expression of Vimentin and Snail and promoted the expression of E-cadherin in a dose-dependent manner. Conclusion The experimental group treated with cinnamaldehyde inhibits the proliferation, migration and invasion of OSCC CAL27 and promotes its apoptosis in a dose-dependent manner in two dimensional conditions in vitro. Cinnamaldehyde inhibits the migration of OSCC CAL27 by affecting the epithelial-mesenchymal transition process.

Key words: cinnamaldehyde, CAL27 cells, proliferation, migration., invasion, apoptosis, EMT

CLC Number:

R739.85

xu jiaqi Qiusheng Shan. The effect of cinnamaldehyde on the proliferation and migration of oral squamous cell carcinoma CAL27 cells[J]. , 2020, 40(10): 888-893.

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The effect of cinnamaldehyde on the proliferation and migration of oral squamous cell carcinoma CAL27 cells

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