Abstract: Objective To analyze the differentially expressed genes and long non-coding RNA (lncRNA) in gene expression microarray of ameloblastoma patients and normal person, and to explore the key lncRNA and its involved signaling pathways. Methods The gene expression omnibus (GEO) microchip data set GES38494 and GES132472 of ameloblastoma were downloaded to screen the differentially expressed genes and lncRNAs. The lncRNA-mRNA co-expression network was constructed and its involved GO and KEGG pathways were analyzed. The STRING database was used for protein interaction analysis and screening of core genes. Results A total of 801 differentially expressed genes were screened out by differential analysis, among which six were differentially expressed lncRNA and co-expressed with 283 mRNAs. The mRNA in the lncRNA-mRNA co-expression network was mainly involved in biological functions such as epithelial development, skin development, epidermal cell differentiation, keratinocyte differentiation and keratinization, as well as the pluripotent regulation pathway of stem cells, pancreatic secretion, retinol metabolism, axon guidance, extracellular matrix - receptor interaction, and adhesion signaling pathway of KEGG. In PPI analysis, IVL was the core gene with the highest degree of connectivity, and the expression of its co-expressed lncRNA MAGI2-AS3 was down-regulation, showing a negative correlation. Conclusion Through bioinformatics analysis, signaling pathways that related to ameloblastoma lncRNA-mRNA co-expression network genes and core genes of protein interaction are found out, which provides a theoretical basis for target selection in ameloblastoma treatment .

Key words: ameloblastoma, differential gene, Long non-coding RNA, co-expression network, signaling pathway