Abstract: [Abstract] Objective: To investigate the effect of Resolvin E1 (RvE1) on the biological performance of human dental pulp stem cells (hDPSCs) under hydrogen peroxide-induced (H2O2) oxidative stress. Methods: The hDPSCS were isolated and cultured in vitro by enzymatic tissue block method, and the expression of RvE1 receptor ChemR23 in hDPSCs was detected by immunofluorescence staining; hDPSCs were randomly divided into 3 groups, control group, H2O2 group and H2O2+RvE1 group, H2O2 group and H2O2+RvE1 group were pretreated with 200μM H2O2 for 24h to establish a model of cell oxidative stress. After 24h, the control group and H2O2 group continued to use complete culture medium, and the H2O2+RvE1 group continued to use complete medium containing 100nMRvE1. Hematoxylin-eosin(HE) staining and phalloidin staining were used to observe the morphological changes of hDPSCs; cck-8, scratch test and alkaline phosphatase (ALP) staining were used to detect the changes of hDPSCs proliferation, migration and odontogenic differentiation ability. Results: ChemR23 was widely expressed in hDPSCs; Compared with the control group, hDPSCs in the H2O2 group had abnormal morphology and the ability of proliferation, migration, and odontogenic differentiation was significantly decreased (P <0.05). Compared with the H2O2 group, hDPSCs in the H2O2+RvE1 group had significantly enhanced ability of proliferation, migration and odontogenic differentiation(P <0.05). Conclusion: RvE1 promotes the proliferation, migration and odontogenic differentiation of hDPSCs under oxidative stress.

Key words: resolvin E1, dental pulp stem cells, oxidative stress