利用粪肠球菌感染根管模型评价不同根管封闭剂的抗菌效果

doi:10.13591/j.cnki.kqyx.2022.01.007

口腔医学 ›› 2022, Vol. 42 ›› Issue (1): 42-46.doi: 10.13591/j.cnki.kqyx.2022.01.007

利用粪肠球菌感染根管模型评价不同根管封闭剂的抗菌效果

李克朋¹, 程甜甜¹, 仇珺², 尤苏霞², 蒋文凯², 梅笑寒²

1 中国人民解放军海军安庆医院口腔科,安徽安庆(246000);
2 军事口腔医学国家重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,第四军医大学口腔医学院牙体牙髓病科,陕西西安(710032)

修回日期:2021-10-18 出版日期:2022-01-28 发布日期:2022-01-28
通讯作者: 梅笑寒 Tel:(029)84772614,E-mail:10472915@qq.com
蒋文凯 Tel:(029)84772614,E-mail:19518923@qq.com
基金资助:
国家自然科学基金(81700951,81803186);创新人才推进计划-青年科技新星项目(2019KJXX-086);陕西省重点研发计划(2020SF-019)

To evaluate the antibacterial ability of different root canal sealers by using the Enterococcus faecalis infected root canal model

LI Kepeng, CHENG Tiantian, QIU Jun, YOU Suxia, JIANG Wenkai, MEI Xiaohan

Department of Stomatology, The PLA Navy AnQing Hospital, Anqing 246000, China

Revised:2021-10-18 Online:2022-01-28 Published:2022-01-28

摘要/Abstract

摘要： 目的体外建立粪肠球菌根管感染模型,比较3种生物陶瓷类根管封闭剂的抗菌性能。方法选择人单直根管的离体前牙48颗,接种粪肠球菌并孵育4周以构建体外根管感染模型。完成根管成形和清理后,随机将样本分为3个实验组和2个对照组,每组按照如下方法进行根管充填:A组,Biodentine+牙胶;B组,iRoot BP+牙胶;C组,MTA+牙胶;D组,阳性对照组(单纯牙胶充填);E组,阴性对照组(无菌根管)。分别于第1、3、7、14天取样,利用涡轮机磨除充填材料和牙本质,收集粉末,梯度稀释后,常规进行细菌培养并计数。结果 3个实验组在4个时间点均表现出对粪肠球菌的抗菌性,与阴性及阳性对照组相比,有统计学意义(P<0.05),3个实验组抗菌效果组间无明显差异(P>0.05)。此外,随时间延长3种材料杀菌效果逐渐增强,第14天达到最高值。结论 Biodentine、iRoot BP、MTA具有相似的抗菌能力,均能有效杀灭粪肠球菌。

关键词: 根管封闭剂, 抗菌效果, 粪肠球菌, 感染根管模型

Abstract: Objective To establish the model of Enterococcus faecalis root canal infection in vitro and to compare the antibacterial properties of three bio-ceramic root canal sealers. Methods Forty-eight isolated anterior teeth of human single straight root canal were inoculated with Enterococcus faecalis and incubated for 4 weeks to construct an in vitro root canal infection model. After shaping and cleaning, samples were randomly divided into three experimental groups and two control groups. Each group was filled according to the following methods: A, Biodentine; B, iRoot BP; C, MTA; D, positive control group (simple cone filling); E, negative control group (sterile root canal). Samples were taken on day 1, 3, 7 and 14 respectively, and the powder after grinding off the filling material and dentin was collected. After gradient dilution, bacteria were cultured and counted. Results Compared with the positive control group, three experimental groups showed antibacterial activity against Enterococcus faecalis during four time points, which was statistically significant (P<0.05), and there was no significant difference among three experimental groups (P>0.05). In addition, germicidal efficacy of the three materials increased gradually with the extension of time, and reached the highest value in the 14^th day. Conclusion Biodentine, iRoot BP and MTA have similar antibacterial properties and can effectively kill Enterococcus faecalis.

Key words: root canal sealer, antibacterial efficacy, Enterococcus faecalis, infected root canal model

中图分类号:

R783.1

李克朋, 程甜甜, 仇珺, 尤苏霞, 蒋文凯, 梅笑寒. 利用粪肠球菌感染根管模型评价不同根管封闭剂的抗菌效果[J]. 口腔医学, 2022, 42(1): 42-46.

LI Kepeng, CHENG Tiantian, QIU Jun, YOU Suxia, JIANG Wenkai, MEI Xiaohan. To evaluate the antibacterial ability of different root canal sealers by using the Enterococcus faecalis infected root canal model[J]. Stomatology, 2022, 42(1): 42-46.

参考文献

[1] Cheng XG, Guan SM, Lu H, et al. Evaluation of the bactericidal effect of Nd: YAG, Er: YAG, Er, Cr: YSGG laser radiation, and antimicrobial photodynamic therapy (aPDT) in experimentally infected root canals[J]. Lasers Surg Med, 2012, 44(10):824-831.
[2] Refaei P, Jahromi MZ, Moughari AAK. Comparison of the microleakage of mineral trioxide aggregate, calcium-enriched mixture cement, and Biodentine orthograde apical plug[J]. Dent Res J (Isfahan), 2020, 17(1):66-72.
[3] 田青鹭, 王艳. 生物陶瓷材料在根尖屏障术中的应用进展[J]. 口腔医学研究, 2021, 37(3):191-194.
[4] Mantri SP, Kapur R, Gupta NA, et al. Type Ⅲ apical transportation of root canal[J]. Contemp Clin Dent, 2012, 3(1):134-136.
[5] Dioguardi M, Alovisi M, Sovereto D,et al. Sealing ability and microbial leakage of root-end filling materials: MTA versus epoxy resin: A systematic review and meta-analysis[J]. Heliyon, 2021, 7(7):e07494.
[6] Mahgoub N, Alqadasi B, Aldhorae K,et al. Comparison between iRoot BP plus (EndoSequence root repair material) and mineral trioxide aggregate as pulp-capping agents: A systematic review[J]. J Int Soc Prev Community Dent, 2019, 9(6):542-552.
[7] Chen XX, Zhang HM, Zhong J, et al. Comparison of indirect pulp treatment and iRoot BP Plus pulpotomy in primary teeth with extremely deep caries: A prospective randomized trial[J]. Clin Oral Investig, 2021, 25(5):3067-3076.
[8] Song WC, Li SE, Tang QM, et al. In vitro biocompatibility and bioactivity of calcium silicate-based bioceramics in endodontics (Review)[J]. Int J Mol Med, 2021, 48:128.
[9] 仇珺, 曹海炜, 田宇, 等. 两种方法比较3种根管封闭剂体外抗菌效果的研究[J]. 牙体牙髓牙周病学杂志, 2015, 25(4):238-243,200.
[10] 张敏, 仇珺, 侯晖, 等. 超声下不同根管冲洗剂组合对感染根管内粪肠球菌杀灭效果的研究[J]. 牙体牙髓牙周病学杂志, 2014, 24(11):649-653.
[11] Selis D, Pande Y, Smoczer C, et al. Cytotoxicity and genotoxicity of a new intracanal medicament, 2-hydroxyisocaproic acid-an in vitro study[J]. J Endod, 2019, 45(5):578-583.
[12] Kadam R, Shashikiran S, Mapara P,et al. Comparative evaluation of antimicrobial efficacy of mineral trioxide aggregate, biodentine, Endo Sequence against bacterial strains of Enterococcus faecalis- an in-vitro study[J]. Jemds, 2020, 9(28):1988-1991.
[13] Nayyar P, Sethi A, Thakur D,et al. Antibacterial effect of silver nanoparticle gel as an intracanal medicament in combination with other medicaments against Enterococcus faecalis: An in vitro study[J]. J Pharm Bioallied Sci, 2021, 13(Suppl 1):S408-S411.
[14] Parolia A, Kumar H, Ramamurthy S, et al. Effect of Propolis nanoparticles against Enterococcus faecalis biofilm in the root canal[J]. Molecules, 2021, 26(3):715.
[15] ElReash AA, Hamama H, Eldars W, et al. Antimicrobial activity and pH measurement of calcium silicate cements versus new bioactive resin composite restorative material[J]. BMC Oral Health, 2019, 19(1):235.
[16] Sherief DI, Fathi MS, Abou El Fadl RK. Antimicrobial properties, compressive strength and fluoride release capacity of essential oil-modified glass ionomer cements-an in vitro study[J]. Clin Oral Investig, 2021, 25(4):1879-1888.
[17] Prada I, Micó-Muñoz P, Giner-Lluesma T, et al. In fluence of microbiology on endodontic failure. Literature review[J]. Med Oral Patol Oral Cir Bucal, 2019, 24(3):e364-e372.
[18] Janini ACP, Bombarda GF, Pelepenko LE, et al. Antimicrobial activity of calcium silicate-based dental materials: A literature review[J]. Antibiotics (Basel), 2021, 10(7):865.
[19] 程小刚, 范晓敏, 鲁红, 等. Er: YAG激光对根管内粪肠球菌杀灭效果的观察研究[J]. 牙体牙髓牙周病学杂志, 2013, 23(2):116-120.
[20] Sismanoglu S, Yildirim-Bilmez Z, Gurcan AT, et al. Influence of intracoronal bleaching agents on the bond strength of MTA cements to composite resin and their surface morphology[J]. Odontology, 2021,8(9):400-413.
[21] Palczewska-Komsa M, Kaczor-Wiankowska K, Nowicka A. New bioactive calcium silicate cement mineral trioxide aggregate repair high plasticity (MTA HP)-A systematic review[J]. Materials (Basel), 2021, 14(16):4573.
[22] Esteki P, Jahromi MZ, Tahmourespour A. In vitro antimicrobial activity of mineral trioxide aggregate, Biodentine, and calcium-enriched mixture cement against Enterococcus faecalis, Streptococcus mutans, and Candida albicans using the agar diffusion technique[J]. Dent Res J (Isfahan), 2021, 18:3-9.
[23] 游月华, 周珊羽, 元博,等. 生物陶瓷材料iRoot BP plus和矿物三氧化聚合体MTA应用于成人恒牙根尖屏障术的临床疗效分析[J]. 实用医学杂志 2021,37(7):869-873.
[24] Shin JH, Ryu JJ, Lee SH. Antimicrobial activity and biocompatibility of the mixture of mineral trioxide aggregate and nitric oxide-releasing compound[J]. J Dent Sci, 2021, 16(1):29-36.
[25] Bossù M, Mancini P, Bruni E, et al. Biocompatibility and antibiofilm properties of calcium silicate-based cements: An in vitro evaluation and report of two clinical cases[J]. Biology, 2021, 10(6):470.
[26] Arandi NZ, Thabet M. Minimal intervention in dentistry: A literature review on biodentine as a bioactive pulp capping material[J]. Biomed Res Int, 2021, 2021: 1-13.
[27] Liu M, He L, Wang H,et al. Comparison of in vitro biocompatibility and antibacterial activity of two calcium silicate-based materials[J]. J Mater Sci Mater Med, 2021, 32(5):52.
[28] Koruyucu M, Topcuoglu N, Tuna EB, et al. An assessment of antibacterial activity of three pulp capping materials on Enterococcus faecalis by a direct contact test: An in vitro study[J]. Eur J Dent, 2015, 9(2):240-245.

利用粪肠球菌感染根管模型评价不同根管封闭剂的抗菌效果

To evaluate the antibacterial ability of different root canal sealers by using the Enterococcus faecalis infected root canal model

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics

本文评价

[1]	崔静雯,范建林. PIPS联合纳米银对根管粪肠球菌抗菌效果研究[J]. 口腔医学, 2023, 43(2): 110-113.
[2]	徐章凤,张昊,周永庆. 单尖法和热牙胶法根管充填根尖封闭性能的比较研究[J]. 口腔医学, 2023, 43(2): 114-117.
[3]	荣迪, 巫慧丽, 王莹, 王晶艳, 张娟, 周广超, 顾培玉, 吴大明. 根管封闭剂和锥形束CT拍摄参数对牙根纵裂诊断的影响[J]. 口腔医学, 2022, 42(6): 529-533.
[4]	王晓娜石晶. 新型饵激光与超声对根管荡洗作用的对比研究[J]. , 2021, 41(4): 328-332.
[5]	玛丽亚木古丽·帕塔尔牛巧丽艾山?依力哈木李丹丹赵今. GuttaFlow对根管充填封闭性能的体外研究[J]. , 2018, 38(2): 124-126.
[6]	樊少昕池学谦. 不同根管封闭剂封闭性的研究进展[J]. , 2018, 38(1): 73-77.
[7]	孙启俊江龙韦晓玲胡济安. 不同根管封闭糊剂在一次性根管治疗中的临床疗效研究[J]. , 2016, 36(10): 890-893.
[8]	王梦婷许玉婷陈亚明. 生物活性玻璃对3种细菌和龈下早期混合菌斑抗菌效果的研究[J]. , 2015, 35(5): 354-358.
[9]	鲁朝阳王小平. 氧化锌丁香油类根管封闭剂粘度对根管封闭性能的影响[J]. , 2015, 35(2): 128-131.
[10]	楼国芳. 3种封闭剂在一次性根管治疗术后的近期疼痛比较[J]. , 2010, 30(12): 734-735.
[11]	查光玉;张加理. 根管封闭剂的临床理化特性[J]. , 2007, 27(7): 380-383.
[12]	冯朝华;沈嵩;高学军. 次氯酸钠对粪肠球菌杀菌效果的体外研究[J]. , 2007, 27(10): 513-515.
[13]	方秋萍,蒋金峰,王维倩,王仁飞. 死髓牙经不同方法根管充填后近期疼痛分析[J]. , 2005, 25(5): 317-318.