小鼠帽状期和钟状早期磨牙牙胚差异表达基因的初探

›› 2013, Vol. 33 ›› Issue (1): 1-4.

小鼠帽状期和钟状早期磨牙牙胚差异表达基因的初探

汪涵¹,陈卓²,³

1. 杭州口腔医院
2. 浙江大学附属口腔医院,
3.

收稿日期:2012-07-23 修回日期:2012-08-27 出版日期:2013-01-29 发布日期:2013-02-05
通讯作者: 汪涵 E-mail:1013s@163.com
基金资助:
国家自然科学基金;浙江省教育厅基金

The primary study on differentially expressed genes of in murine molar germ at cap stage and early bell stage

Han WANG ²,³

Received:2012-07-23 Revised:2012-08-27 Online:2013-01-29 Published:2013-02-05
Contact: Han WANG E-mail:1013s@163.com

摘要/Abstract

摘要： 目的筛选小鼠帽状期（E14.5）和钟状早期（E18.5）磨牙牙胚的差异表达基因。方法分离小鼠帽状期（E14.5）和钟状早期（E18.5）磨牙牙胚，采用基因芯片技术检测相关的差异表达基因。结果共得到76条差异表达基因，上调基因71条，下调基因5条。结论在钟状早期（E18.5）磨牙牙胚，调控细胞代谢、分化、极性形成酶类，调控免疫应答相关基因，转录、翻译相关基因，信号通路的信号分子、受体以及应激反应相关基因等功能的基因表达都显著上调，而细胞凋亡相关基因、氨基酸代谢激酶等的表达下调。

关键词: 牙胚发育, 帽状期, 钟状早期, 差异表达基因, tooth development, cap stage, early bell stage, different gene expressions

Abstract: Objective To study the different gene expressions of cap stage (E14.5) and early bell stage (E18.5) in murine molar. Methods The mouse molar germ at E14.5 and E18.5 were removed. Gene chip was used to obtain the different gene expressions. Results There were 76 differently expressed genes, 71 up-regulated genes and 5 down-regulated genes. Conclusion In molar germ of early bell stage (E18.5), genes related to cell metabolism, differentiation, formation of polarity, regulating immune response, regulating transcription and translation, signaling molecules and receptors, stress response are up-regulated dramatically. Genes related to cell apoptosis and amino acid metabolism kinases are down-regulated.

中图分类号:

R349.64

汪涵陈卓. 小鼠帽状期和钟状早期磨牙牙胚差异表达基因的初探[J]. 口腔医学, 2013, 33(1): 1-4.

Han WANG. The primary study on differentially expressed genes of in murine molar germ at cap stage and early bell stage [J]. Stomatology, 2013, 33(1): 1-4.

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