关键词: 氟, 成釉细胞, 骨组织, 大鼠, fluoride, ameloblast, bone tissues, rats

Abstract: To establish the animal model of drinking water type fluorosis and observe the morphological changes of its sclerous tissues. Methods 48 Wistar rats were randomly divided into four groups.The fluoride exposed groups respectively drank water containing 50,100 and 150 mg/L NaF,and the control group drank ordinary tap water.8 weeks later,incisors and femur of rats were taken and HematoxylinEosin(HE) staining method was used to observe the changes in rat incisor ameloblasts and the morphological change of bone tissues.The concentrations of fluoride in serum,teeth and bone were determined by fluorine ion selective electrode. Results The rat incisors in the experimental groups showed different performances of dental fluorosis.The contents of fluoride in serum（F=11.234，P<0.05），teeth(F=275.148，P<0.05 ) and bone(F=217.337，P<0.05) in the fluoride exposed groups were much higher than those of the control group.It increased with the increase of concentration,and it showed significant differences among four groups.According to HE staining,in experimental groups,the irregular arrangement of the rat incisor ameloblasts was multilayered.The columnar cells were getting shorter or disappeared,and a few cells were distorted.With the increase of fluoride concentration,the pathological changes got more serious.HE staining showed that the bone trabecula increased and closely arranged together in rat bone tissues in experimental group,which indicated bone sclerosis.Also,epiphyseal plate was getting thicker and the hypertrophic chondrocytes accumulated and arranged disorderedly in fluoridetreated rats.Conclusions Drinking water containing high concentration of fluoride for long time may cause chronic fluorine poisoning and pathological changes of sclerous tissues such as dental fluorosis and skeletal fluorosis.