›› 2013, Vol. 33 ›› Issue (11): 729-732.

• 基础与临床研究 • 上一篇    下一篇

3种种植用钛合金铸造前后的细胞毒性研究

赵惠1,李潇2,金柱坤2,3,杨凯2,3   

  1. 1. 广州军区广州总医院
    2. 广州军区总医院
    3.
  • 收稿日期:2013-03-04 修回日期:2013-07-03 出版日期:2013-11-28 发布日期:2013-11-25
  • 通讯作者: 李潇 E-mail:drlixiao@163.com
  • 基金资助:
    全军医学科研“十二五”面上课题;广东省产学研结合项目

The cytotoxicity of three kinds of titanium alloys before and after casting

  • Received:2013-03-04 Revised:2013-07-03 Online:2013-11-28 Published:2013-11-25

摘要: 目的 通过细胞培养MTT(噻唑蓝)实验,评价铸造对钛合金生物相容性的影响。方法分别将3组钛合金铸造前后的材料加工制成直径13.5 mm,厚度1 mm的圆片,清洗消毒后制备浸提液培养细胞,培养1,3,5 d后分别观察细胞形态、测试各组细胞吸光度值。结果①铸造前与铸造后,3组材料细胞吸光度值比较,含铝、钒的C组比其他两组稍低(P>0.05);②3组材料铸造前后吸光度值比较,铸造后稍低于铸造前(P>0.05)。结论铸造对于钛合金生物相容性无影响,不影响临床应用。

关键词: 铸造, 种植, 细胞毒性, 钛, cytotoxicity, titanium alloy

Abstract: Abstract:Objective To evaluate the biocompatibility of titanium alloy before and after casting by cell culture and MTT test. Methods Three kinds of titanium alloys before and after casting were processed into wafers whose diameter was 13.5 mm and thickness was 1 mm.Extracts were prepared for cell culture after cleaning and disinfection.Observe cell morphology and test the cells′ absorbance value in each group after culturing for 1,3 and 5 days. Results ①Cell absorbance value of C group which contained aluminum and vanadium was slightly lower than those of the other two groups no matter before or after casting(P>0.05).②Cell absorbance values of three groups after casting were slightly lower than those before casting (P>0.05). Conclusions Casting has no effect on the biocompatibility of titanium alloys.It doesn′t affect clinical application.

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