关键词: 基因转染, BMP-2, 壳聚糖, 骨髓基质干细胞, 成骨样细胞, 碱性磷酸酶, 护骨素, 骨钙素, 骨桥蛋白, gene transfection, BMP-2, chitosan, bone marrow stem cells, osteoblast-like cells, alkaline phosphatase, Osteoprotegerin, gingival crevicular fluid, Osteopontin

Abstract: Objective To investigate expressions of genes in osteoblast-like cells derived from bone marrow stem cells (BMSCs) transfected with chitosan nanocarrier containing plasmid-BMP-2 sequences (CNPBs). Methods According to different concentrations of plasmid–BMP-2 (pBMP-2), CNPBs experimental groups were divided into CPB50 concentration group (50 μg/mL pBMP-2), CPB100 concentration group (100 μg/mL pBMP-2) and CPB200 concentration group (200 μg/mL pBMP-2) respectively. Expression levels of specific molecules (Alkaline Phosphatase, ALP; Osteoprotegerin, OPG; Osteocalcin, OC; Osteopontin, OPN) in osteoblast-like cells were detected and analyzed. Results There were higher expression levels of ALP, OPG, OC and OPN genes in osteoblast-like cells in CNPBs groups than control groups, especially in CBP200 group. Conclusion Expression levels of genes in osteoblast-like cells derived from BMSCs transfected with CNPBs can be promoted effectively.