人牙周膜干细胞的成脂诱导实验研究

›› 2013, Vol. 33 ›› Issue (4): 254-257.

人牙周膜干细胞的成脂诱导实验研究

钟志华¹,鄢少君²,周先略¹,贺国权¹,何爱萍¹

1. 荆州市中心医院
2. 长江大学医学院口腔科

收稿日期:2012-03-02 修回日期:2012-04-16 出版日期:2013-04-28 发布日期:2013-04-18
通讯作者: 钟志华 E-mail:xz737@163.com
基金资助:
湖北省卫生厅科研项目

Experiment on inducing human periodontal ligament stem cells into adipocytes

Received:2012-03-02 Revised:2012-04-16 Online:2013-04-28 Published:2013-04-18

摘要/Abstract

摘要： 目的体外分离培养成人的牙周膜干细胞(hPDLSC，human Periodontal Ligament Stem Cells)，做成脂诱导实验。方法取新鲜拔除正畸牙的牙周膜为材料制成组织块，加入培养液分离培养，免疫磁珠法筛选人牙周膜干细胞，加入成脂诱导液诱导牙周膜干细胞向脂肪细胞转化。结果成功筛选人牙周膜干细胞，成脂诱导成功。结论免疫磁珠法是筛选人牙周膜干细胞的有效方法，人牙周膜干细胞可以向脂肪细胞分化。

关键词: 人牙周膜干细胞, 免疫磁珠, 成脂诱导, human periodontal ligament stem cells(hPDLSC), immunomagnetic beads, adipogenic induction

Abstract: Objective To induce the periodontal ligament stem cells in vitro into adipocytes．Methods Periodontal ligament tissue was obtained from extracted orthodontal teeth, then cultivated in nutrient solution. hPDLSC was selected with immunomagnetic beads method. hPDLSC was induced into adipocytes in the adipogenic nutrient solution. Results hPDLSC was selected successfully with immunomagnetic beads method. hPDLSC was induced into adipocytes successfully.Conclusion Immunomagnetic beads method is an effective way to isolate and purify the hPDLSC and hPDLSC was able to transfer into adipocyte.

中图分类号:

R349.5

钟志华鄢少君周先略贺国权何爱萍. 人牙周膜干细胞的成脂诱导实验研究[J]. 口腔医学, 2013, 33(4): 254-257.

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[2]	周益翔刘根霞马姝卢亚蝶张光东于金华. MTA对牙周膜干细胞RANKL/OPG表达水平的影响[J]. , 2015, 35(8): 619-622.