›› 2014, Vol. 34 ›› Issue (1): 13-15.

• 基础与临床研究 • 上一篇    下一篇

右旋氨基酸对牙龈卟啉单胞菌生物膜形成的影响

李琛1,潘亚萍2,林莉1,刘静波1,郭艳2,3   

  1. 1. 中国医科大学附属口腔医院
    2. 中国医科大学口腔医学院
    3.
  • 收稿日期:2013-06-26 修回日期:2013-08-22 出版日期:2014-01-28 发布日期:2014-01-28
  • 通讯作者: 潘亚萍 E-mail:yppan@mail.cmu.edu.cn
  • 基金资助:
    唾液酸酶基因的缺失影响牙龈卟啉单胞菌致病性的机制研究;p21蛋白修饰对牙龈卟啉单胞菌内化上皮细胞凋亡和周期调控的研究;唾液酸酶基因的缺失影响牙龈卟啉单胞菌致病性的机制研究

The effect of D-animo acid on Porphyromonas gingivalis biofilm formation

  • Received:2013-06-26 Revised:2013-08-22 Online:2014-01-28 Published:2014-01-28

摘要: 目的 本实验研究右旋色氨酸、右旋亮氨酸、右旋酪氨酸和右旋蛋氨酸对牙龈卟啉单胞菌生物膜形成的影响。方法 用含有维生素K(1 μg/mL)和氯化血红素(5 μg/mL)的TSB培养基培养牙龈卟啉单胞菌W83,培养到对数生长期时1:100倍稀释后放入96孔板中,分别加入右旋色氨酸(5 mmol/L)、右旋亮氨酸(8.5 mmol/L)、右旋酪氨酸(3 μmol/L)和右旋蛋氨酸(2 mmol/L),厌氧培养7 d后结晶紫染色,双蒸水冲洗,干燥后乙醇脱色,紫外分光光度计检测。 结果 加入右旋色氨酸、右旋亮氨酸、右旋酪氨酸和右旋蛋氨酸及空白对照组的吸收值分别为3.70 ± 0.06, 2.74 ± 0.18, 0.71 ± 0.04,3.42 ± 0.02,和3.65 ± 0.01。与空白对照组相比较,加入右旋亮氨酸组吸收值小,并有统计学差异(P < 0.01)。 结论 右旋亮氨酸能够减少牙龈卟啉单胞菌生物膜形成。

关键词: 右旋氨基酸, 牙龈卟啉单胞菌, 生物膜, D-animo acid, Porphyromonas gingivalis, biofilm formation

Abstract: Objective To study the effect of D-Tryptophan, D-Leucine, D-tyrosine, and D-Methionine on the formation of biofilm Porphyromonas gingivalis (P. gingivalis) W83. Method: P. gingivalis W83 were cultured in Trypticase soy broth (TSB) supplemented with vitamin K (1μg/ml) and hemin (5 μg/ml) to mid-log phase, and then were diluted 1:100 in 96 well flat-bottom poltstyrene plates and add D-Tryptophan (5mmol/L), D-Leucine (8.5 mmol/L), D-tyrosine (3μmol/L), and D-Methionine (2mmol/L). The biofilms were stained with 25μl of 1% crystal violet, washed with ddH2O and dissolved in ethanol. The absorbance was measured with xMark microplate spectrophotometer at 570nm. Result: The absorbance of P. gingivalis W83 in TSB, D-Tryptophan, D-Leucine, D-tyrosine, and D-Methionine were 3.70 ± 0.06, 2.74 ± 0.18, 0.71 ± 0.04, 3.42 ± 0.02, and 3.65 ± 0.01 respectively. There was significance difference between TSB and D-Leucine groups (p<0.01). Conclusion D-Leucine (8.5 mmol/L) can reduce the biofilm formation of P. gingivalis W83.

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