›› 2014, Vol. 34 ›› Issue (11): 801-805.

• 基础与临床研究 •    下一篇

脂联素对不同培养条件下成骨细胞与间充质干细胞中 SDF-1/CXCR4 表达影响的实验研究

於丽明1,陆萌萌1,罗远1,涂旗胜2,陈锦坤2   

  1. 1. 上海市口腔病防治院
    2. Tufts University School of Dental Medicine
  • 收稿日期:2014-03-28 修回日期:2014-05-03 出版日期:2014-11-28 发布日期:2014-12-03
  • 通讯作者: 於丽明 E-mail:ylmphoebe@126.com
  • 基金资助:
    上海市自然科学基金青年项目;上海市卫生局青年科研项目

Adiponectin Regulates SDF-1/CXCR4 Expression of Osteoblasts and Mesenchymal Stem Cells in Different Culture Systems

  • Received:2014-03-28 Revised:2014-05-03 Online:2014-11-28 Published:2014-12-03

摘要: 目的 研究不同培养条件下,脂联素对成骨细胞的基质细胞衍生因子(SDF-1)以及间充质干细胞 CXCR4表达的影响。方法 提取重组的脂联素蛋白,将10 μg/mL脂联素作用于单独培养的MC3T3-E1以及C3H10T1/2细胞, Real-time定量PCR方法检测SDF-1/CXCR4表达变化;设计MC3T3-E1及C3H10T1/2细胞共培养体系,比较共培养对MC3T3-E1及C3H10T1/2细胞的SDF-1/CXCR4表达变化;将脂联素加入共培养体系,比较脂联素对共培养体系中MC3T3-E1及C3H10T1/2细胞SDF-1/CXCR4 mRNA表达变化。 结果 Real-time定量PCR结果显示:单独培养条件下,脂联素促进了MC3T3-E1细胞SDF-1mRNA的表达;而在共培养体系中,脂联素则下调了MC3T3-E1细胞SDF-1mRNA的表达。无论单独培养还是共同培养,脂联素对C3H10T1/2细胞的CXCR4表达均起下调作用。结论 脂联素对成骨细胞SDF-1的表达呈双向调节作用,而对间充质干细胞CXCR4则起下调作用。脂联素通过调节SDF-1/CXCR4影响间充质干细胞和成骨细胞的微环境。

关键词: 脂联素, 基质细胞衍生因子, CXCR4, 共培养

Abstract: Objective The present study aims to determine the effect of adiponectin on SDF-1/CXCR4 expression of MC3T3-E1 and C3H10T1/2 cells within different culture systems. Methods Cultured MC3T3-E1 and C3H10T1/2 cells were treated with or without adiponectin, respectively. Real-time quantitative PCR (qPCR) was used to detect SDF-1 and CXCR4 mRNA expressions. Then, we designed a co-culture system with MC3T3-E1 cells in upper chamber and C3H10T1/2 cells in lower chamber. After adiponectin treatment, cells were collected and SDF-1/CXCR4 mRNA expressions were examined. Results qPCR results showed that adiponectin increased SDF-1 mRNA expression when MC3T3-E1 cells cultured alone, while decreased SDF-1 mRNA expression when MC3T3-E1 cells cultured in co-culture system. Adiponectin inhibit CXCR4 mRNA expression in C3H10T1/2 cells, regardless of culture conditions. Conclusion We concluded that adiponectin has dual effects on SDF-1 expression of MC3T3-E1 cells. Adiponectin influences mesenchymal stem cells and osteoblasts niche by regulating SDF-1/CXCR4 axis.

Key words: Adiponectin, SDF-1, CXCR4, Cell Co-culture

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