›› 2014, Vol. 34 ›› Issue (12): 889-891.

• 基础与临床研究 • 上一篇    下一篇

产气荚膜梭菌肠毒素对鼠颌下腺紧密连接蛋白表达影响的研究

李宏卫1,祁兵2   

  1. 1. 南京医科大学附属口腔医院
    2. 南京医科大学附属口腔医院病理科
  • 收稿日期:2014-07-21 修回日期:2014-08-27 出版日期:2014-12-28 发布日期:2015-01-02
  • 通讯作者: 祁兵 E-mail:qb_njmu@sina.com
  • 基金资助:
    教育部留学回国人员科研启动基金资助项目

Effect of Clostridium perfringens enterotoxin on expression of tight junction proteins in rat submandibular glands

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  • Received:2014-07-21 Revised:2014-08-27 Online:2014-12-28 Published:2015-01-02

摘要: 目的 观察产气荚膜杆菌肠毒素对鼠颌下腺上皮细胞形态及细胞间紧密连接蛋白表达影响。 方法 制备提纯气荚膜杆菌肠毒素羧基段184-319残基短肽片段,并灌注大鼠颌下腺3h,行H.E及免疫荧光组织化学染色,观察腺泡及导管细胞形态及紧密蛋白ZO-1及Claudin-4表达变化。 结果 成功提纯产气荚膜杆菌肠毒素短肽片段,未观测到上皮坏死,未观察到紧密连接ZO-1及Claudin-4表达变化。 结论 未发现产气荚膜杆菌肠毒素短肽片段对上皮细胞及其细胞间紧密连接的破坏作用,为进一步研究细胞间涎腺分泌机理提供基础。

关键词: 产气荚膜杆菌, 毒素, 紧密连接, 颌下腺

Abstract: objective To observe the effect of Clostridium perfringens enterotoxin on expression of tight junction proteins in rat submandibular glands Methods recombinant plasmid pET16bHis10C-CPE was constructed, CPE COOH-terminal fragments (C-CPE) were purified. Rat submandibular glands (SMGs) were isolated and then perfused with C-CPE for 3h. After perfusion, the morphology of epithelia cells and expression of ZO-1 and Claudin-4 in SMGs were observed by H.E and immunofluorescence staining. Results After perfusion with C-CPE, no epithelial cells damage was observed and the expression of ZO-1 and Claudin-4 was no change. Conclusions Purified C-CPE fragments have no cytotoxic effect on epithelial cells in SMGs.

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