钛合金种植体表面生物活性因子缓释涂层的构建及生物学评价

摘要/Abstract

摘要： 目的探讨在纯钛及其合金种植体表面构建生物活性因子缓释涂层的可行途径。方法通过模拟体液仿生沉积法在钛合金种植体表面构建钙磷离子/神经生长因子(nerve growth factor，NGF)复合涂层，扫描电镜观察表面形貌、检测厚度,声发射划痕仪检测结合强度； ELISA法检测涂层所含NGF的总量及体外缓释规律；倒置相差显微镜观察释出的NGF对大鼠骨髓间充质干细胞定向成骨细胞分化诱导的影响。结果本实验制备的复合涂层具有粗糙的表面，平均厚度为（11.2±0.26）μm，平均临界载荷为（6.8±0.37）N；所含的NGF可在体外缓释2周以上，并具有生物活性。结论模拟体液仿生沉积法可在钛合金种植体表面成功构建具有缓释生物活性因子能力的复合涂层，其释出的NGF可促进成骨。

关键词: 钛, 种植体, 骨整合, 神经生长因子, 复合涂层

Abstract: Objective To explore the feasible way of constructing a bioactive factor release coating on the surface of titanium alloy implant and to investigate the bioactivity of a calcium/phosphorus/nerve growth factor composite coating constructed on the surface of titanium alloy implant. Methods Calcium/phosphorus/nerve growth factor composite coatings were prepared on the surface of titanium alloy implants using biomimetic deposition,and then characterized by Scanning Electron Microscope,and an automatic scratch tester.The total content of NGF in the composite coatings and the release value were measured every week by ELISA.The formation of calcified nodules was detected using a phase-contrast microscope. Results The coatings have rough surface,uniform and moderate thickness,and appropriate bonding strength.NGF can be released in vitro for at least 2 weeks,and remains active. Conclusions The calcium/phosphorus/nerve growth factor composite coatings prepared by biomimetic deposition can meet the demands of experimental research and clinical application.

Key words: Titanium, Implant, Osseointegration, Nerve growth factor, Composite coating

中图分类号:

R783.1

吴雪林何浩刘柳黄浩原陈科屹牟雁东. 钛合金种植体表面生物活性因子缓释涂层的构建及生物学评价[J]. 口腔医学, 2014, 34(6): 417-422.

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