免疫磁珠筛选大鼠牙髓干细胞、外胚间充质干细胞的表型研究

›› 2014, Vol. 34 ›› Issue (6): 454-456.

免疫磁珠筛选大鼠牙髓干细胞、外胚间充质干细胞的表型研究

王亦菁¹,张晓东²,孙淏海¹,金岩³,史俊南⁴

1. 沈阳军区总医院口腔内科门诊
2. 沈阳军区总医院
3. 第四军医大学口腔医学院组织工程中心
4. 第四军医大学口腔医学院牙体牙髓科

收稿日期:2013-09-03 修回日期:2013-12-17 出版日期:2014-06-28 发布日期:2014-07-07
通讯作者: 王亦菁 E-mail:kpaba@sina.com.cn

Phenotypic study of rat dental stem pulp cell and ectomesenchymal stem cell screened by immunomagnetic bead

Received:2013-09-03 Revised:2013-12-17 Online:2014-06-28 Published:2014-07-07

摘要/Abstract

摘要： 目的检测磁珠分离细胞STRO-1+的DPSC、EMSC中HNK-1、Nestin的表达，进一步了解DPSC的表型特点及与EMSC的相关性，为今后研究提供较为纯化的细胞来源。方法采用间接免疫磁珠分离法获得DPSC、EMSC，间接免疫荧光双标法检测抗原HNK-1、Nestin的表达。结果磁珠分离前后进行细胞计数，大约有5％的DPSC为 STRO-1+的细胞，大约有1％的EMSC为STRO-1+的细胞；STRO-1+的DPSC免疫荧光检测同时表达HNK-1（++）、Nestin(+)，STRO-1+的EMSC免疫荧光检测显示也同时表达HNK-1（++）、Nestin（++）。结论免疫磁珠分离法获得STRO-1+阳性的DPSC共表达EMSC的标记HNK-1和Nestin，进一步说明二者作为间充质来源的干细胞，细胞表型具有继承性。

关键词: 疫磁珠分离, 牙髓干细胞, 外胚间充质干细胞, STRO-1, HNK-1, Nestin

Abstract: Objective The expression of HNK-1 and Nestin will be checked in the STRO positive DPSC and EMSC which are separated by immunomagnetic bead.To investigate the phenotype characteristics of DPSC and the it s correlation with EMSC to find out the purified cell source for the future study. Methods DPSC and EMSC were obtained with the indirect immunomagnetic bead seperation method.The expression of HNK-1 and Nestin was checked with the indirect immunofluorescent double marker. Results Cell counting was made before and after immunomagnetic bead separation.About 5% DPSCs and 1% EMSCs were STRO-1 positive.HNK-1 was simultaneously expressed in STRO-1 positive DPSC.HNK-1 and Nestin were simultaneously expressed in STRO-1 positive EMSC. Conclusions The STRO-1 positive DPSC expressed the EMSC marker of HNK-1 and Nestin,which shows that both of them are the stem cells from the mesenchymal tissue and the phenotype has the inheritance.

Key words: immunomagnetic bead separation, DPSC, EMSC, STRO-1, HNK-1, Nestin

中图分类号:

R392.12

王亦菁张晓东孙淏海金岩史俊南. 免疫磁珠筛选大鼠牙髓干细胞、外胚间充质干细胞的表型研究[J]. 口腔医学, 2014, 34(6): 454-456.

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