骨桥蛋白影响矿化液诱导牙髓干细胞成骨分化能力的研究

›› 2014, Vol. 34 ›› Issue (8): 566-569.

骨桥蛋白影响矿化液诱导牙髓干细胞成骨分化能力的研究

史欣¹,张鹏飞¹,孙玉芬²,袁梦桐¹,刘明月¹,胡伟平¹

1. 哈尔滨医科大学附属第二医院
2. 黑龙江省黑力医院

收稿日期:2013-12-20 修回日期:2014-01-24 出版日期:2014-08-28 发布日期:2014-09-25
通讯作者: 胡伟平 E-mail:huweiping1963@163.com
基金资助:
骨桥蛋白与骨涎蛋白促进人牙髓干细胞成骨分化的旁分泌作用

Research about the influence of Osteopontin (OPN) on osteogenetic differentiation ability of dental pulp stem cells (DPSCs) induced by mineralizing culture medium

Received:2013-12-20 Revised:2014-01-24 Online:2014-08-28 Published:2014-09-25

摘要/Abstract

摘要： 目的　研究骨桥蛋白（Osteopontin,OPN）对矿化液诱导牙髓干细胞(dental pulp stem cells,DPSCs)成骨分化能力的影响,并优化其诱导条件。方法　矿化液培养DPSCs作为对照组，在矿化液培养基础上添加适宜浓度OPN作为实验组，在倒置相差显微镜下观察诱导后的DPSCs形态变化；应用茜素红染色法检测矿化结节的形成；采用反转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-RCR)分别检测DPSCs骨涎蛋白(BSP)、Runt相关转录因子2(Runx-2)、骨钙蛋白(OCN)、Ⅰ型胶原(Col-1)等骨源性基因表达情况。结果　茜素红染色显示诱导培养28d后两组均出现矿化结节,且实验组的数量和大小明显高于对照组。培养7d后两组DPSCs骨源性基因表达均成阳性，且实验组中BSP 、Runx-2、Col-1及OCN等基因表达水平均明显高于对照组。结论　OPN能增强矿化液诱导DPSCs成骨分化的能力。

关键词: 骨桥蛋白, 矿化液, 牙髓干细胞, 分化

Abstract: Objective　To study the influence of Osteopontin (OPN) on osteogenetic differentiation ability of dental pulp stem cells (DPSCs) induced by mineralizing culture medium, and to optimize its inducement conditions. Methods　DPSCs cultured in mineralizing culture medium worked as control group, and DPSCs cultured in mineralizing culture medium with OPN was the experimental group. The morphological changes of DPSCs were observed by the inverted phase contrast microscope; and the formation of mineralize nodules was detected with Alizarin red staining method; the expression levels of osteogenic related genes including bone sialoprotein (BSP), Runt-related transcription factor 2 (Runx-2), osteocalcin (OCN) and collagen-1 (Col-1) were detected by reverse transcription polymerase chain reaction (RT-RCR). Results　Alizarin red staining showed that mineralized nodules have formed after induced for 28 days in both groups, and the number and size of the mineralized nodules in experimental group was obviously larger than that in control group. The expressions of osteogenic related genes were all positive in both groups after 7 days. Expression levels of BSP, Runx - 2, Col-1and OCN genes of experimental group were significantly higher than control group. Conclusions　OPN can enhance the osteogenetic differentiation ability of dental pulp stem cells (DPSCs) induced by mineralizing culture medium.

Key words: osteopontin, mineralizing culture medium, dental pulp stem cells, differentiation

中图分类号:

R329.28

史欣张鹏飞孙玉芬袁梦桐刘明月胡伟平. 骨桥蛋白影响矿化液诱导牙髓干细胞成骨分化能力的研究[J]. 口腔医学, 2014, 34(8): 566-569.

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