细胞角蛋白在牙龈鳞癌颈淋巴结中的表达及临床意义

›› 2014, Vol. 34 ›› Issue (8): 570-572.

细胞角蛋白在牙龈鳞癌颈淋巴结中的表达及临床意义

岳琨¹,贾暮云²,段青云³,袁荣涛¹,王科¹,卜令学¹

1. 青岛大学附属医院
2. 青岛大学医学院附属医院口腔颌面外科
3. 浙江省杭州市第一人民医院

收稿日期:2013-12-17 修回日期:2014-02-21 出版日期:2014-08-28 发布日期:2014-09-25
通讯作者: 岳琨 E-mail:skyspace1128@163.com

Cytokeratin expression in cervical lymph nodes of patients with squamous cell carcinoma of the gingival

Received:2013-12-17 Revised:2014-02-21 Online:2014-08-28 Published:2014-09-25
Contact: Kun YUE E-mail:skyspace1128@163.com

摘要/Abstract

摘要： 目的　研究细胞角蛋白（CK-AE1/AE3）在牙龈鳞状细胞癌颈淋巴结中的表达及临床意义。方法　将17例牙龈鳞癌患者病理报告为阳性的28枚淋巴结及139枚阴性淋巴结重新切片，应用广谱细胞角蛋白单克隆抗体（AE1/AE3）作为免疫标志物，采用免疫组织化学（SP法）检测抗体在淋巴结中的表达。结果　167枚淋巴结中，28枚HE染色阳性的淋巴结CK表达均为阳性；另有11枚HE染色阴性淋巴结CK表达为阳性，其位于Ⅰ区6枚，Ⅱ区5枚，共分布在9例患者中。结论　采用CK（AE1/AE3）作为免疫标志物的免疫组化法检测牙龈鳞状细胞癌的淋巴结，较传统HE染色法更为准确，有利于早期发现微转移灶，从而为牙龈癌的治疗及预后判断提供依据。

关键词: 细胞角蛋白, 牙龈鳞状细胞癌, 淋巴结, 微转移

Abstract: Objective　 To study the expression of cytokeratin in cervical lymph nodes of patients with squamous cell carcinoma of the gingival .Methods: Immunohistochemical examination with pan-cytokeratin (CK-AE1/AE3) was performed on 28 positive lymph nodes and 139 negative lymph nodes that were from 17 patients with squamous cell carcinoma of gingival.Results:28 lymph nodes with metastasis shown by general pathological examination (HE stain) were positive for CK. 11 lymph nodes（in 9 patients） that were shown to have no metastasis by general examination were also positive for CK and 6 nodes were found in level Ⅰand 5 nodes were found in level Ⅱ.Conclusion: The immynohistochemical with pan-cytokeratin (CK-AE1/AE3) is a good method of detecting lymph node micrometastasis in squamous cell carcinoma of the gingival.

Key words: cytokeratin, gingival, lymph nodes, micrometastasis

岳琨贾暮云段青云袁荣涛王科卜令学. 细胞角蛋白在牙龈鳞癌颈淋巴结中的表达及临床意义[J]. 口腔医学, 2014, 34(8): 570-572.

参考文献

[1]孙宁，尤云峰.恶性肿瘤微转移的临床研究[J].临床肿瘤学志,2004,9(2):208-214
[2]李伟忠，丁彦青，赵彤，等.细胞角蛋白在口腔鳞癌颈淋巴结中的表达及意义[J].实用口腔医学杂志,2006,22(6):856-858
[3]Woolgar JA，Beime JC，Vaughan ED，et al.Correlation of histopathological findings wit clinical and radiological assessments of cervical lymph node metastases in oral cancer[J].Oral Maxillofacilal Surg,1995,24(1):30-35
[4]Vander BrekelMW，StelHV，Vander Valk P，et al.Micrometastases from squamous cell carcinoma in neck dissection specinens[J].EurArch Otorhino Laryngo,1992,249(6):349-353
[5]Funanki NO.Tanaka JOhshin G，et alGytokeratin 20 RNA in peripheral venous blood of colorectal carcinoma patients[J]..Br J cancer,1998,77(8):1327-1332
[6]舒萍，鲁大鹏.细胞角蛋白19mRNA在口腔鳞癌侵袭转移中表达的意义[J].北京口腔医学,2009,17(3):128-131
[7]Jung YS,Lee KJ,Kim HJ,et al.Clinical Significance of Bone Marrow Micrometastasis Detects by Nested RT-PCR for keratin 19 in Breast Cancer Patients[J].Jpn J Clin Oncol,2003,33(4):167-172
[8]钱晓云，章宜芬，俞晨杰，等.喉癌及喉咽癌颈部淋巴结微转移的检测及临床意义初探[J].山东大学耳鼻喉眼学报,2010,24(3):9-14
[9]Nakajo A,Natsugoe S,Ishigami S,et al.Detectin and prediction of micrometastasis in the lymph node of patients with pN0 gastric cancer[J].Ann Surg Oncol,2001,8(2):158-162
[10]聂敏海，钟利，曾光明，等.细胞角蛋白CK19在口腔黏膜癌变过程中的变化[J].中华口腔医学杂志,2002,37(3):187-190
[11]Miyake K，Seshimo，Kameoka S.Assessment of lymph node micrometastases in early gastric cancer in relation to sentinel nodes[J].Gastric Cancer,2006,9(3):197-202
[12]Gipponi M，Canavese G，Lionetto R，et al.The role of axilary lymph node dissection in breast cancer patients with sentinel lymph node micrometastases[J].Eur J Surg Oncol,2006,32(2):143-147
[13]Fleming FJ，Hayanga AJ，Glynn F，et al.Incidence and prognostic influence of lymph node micrometastases in rectal cancer[J].Eur J Surg Oncol,2007,33(8):998-1002
[14]Hamakawa H，Takemura K，Sumida T，et al.Histological study on pN upgrading of oral cancer[J].Virchows Arch,2000,437(2):116-121
[15]Barrera JE，Miller ME，Said S,et al.Detection of occult cervical micrometastases in patients with head and neck squamous cell cancer[J].Laryngoscrope,2003,113(5):892-
[16]Ferlito A,Rinaldo A,Devaney KO,et al.Prognostic significance of microscopic andmacroscopic extracapsular spread frommetastatic tumor in the cervical lymph nodes[J].Oral Oncology,2002,38(8):747-751
[17]Kolli VR,Datta RV,Orner JB,et al.The role of supraomohyoid neck dissection in patients with positive nodes[J].Arch Otolaryngol Head Neck Surg,2000,126(3):423-426