再生的牙周组织在正畸压应力下破骨相关因子表达的研究

摘要/Abstract

摘要： 目的研究应用改良的双相磷酸钙(biaphasic calcium phosphate,BCP)复合骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)行犬牙周组织再生后，对再生的牙周组织施加正畸压应力，比较与健康牙周组织受力后破骨相关因子表达的异同，从而探讨牙周再生后进行正畸牙移动的可行性。方法取6只成年雄性比格犬，拔除其双侧上下颌第一前磨牙，随机分为1周组、2周组和4周组，每组2只犬，实验组于每只犬右上、左下象限第二前磨牙牙根的近中侧制造牙周缺损，并将体外培养的犬BMSCs与BCP的共培物植入缺损区，牙周再生20周后施加正畸力，以1.47 N左右的力值拉第二前磨牙向近中方向移动；以自身为对照，另两象限交替作为牙周再生术后加力0周组和空白对照组。分别于加力1周、2周及4周后处死2只比格犬并取组织块。通过Real-Time PCR及Western-Blot印迹法检测破骨相关因子活化T细胞转化因子-1（NFATc-1）和组织蛋白酶K（Cath-K）的表达。结果实验组NFATc-1和Cath-K的mRNA和蛋白表达趋势一致:从0周到2周呈上升趋势，2周至4周下降，但仍高于0周时；实验组与对照组阳性表达情况无明显差异（P>0.05)。结论再生的牙周组织中破骨细胞相关因子的表达情况及变化趋势与正常牙周组织相似，应用改良的BCP用于牙周组织缺损的再生及再生后的正畸牙移动是可行的。

关键词: 双相磷酸钙, 牙周再生, 破骨细胞, NFATc-1, Cath-K, 比格犬

Abstract: Objective To compare the difference of the osteoclastic factors expression between the regenerated periodontal tissue and the healthy periodontal tissue under orthodontic compressional stress. The periodontal tissue was regenerated with the modified biaphasic calcium phosphate(BCP) as scaffold and bone marrow mesenchymal stem cells(BMSCs) as seed cells. To further investigate the possibility of orthodontic tooth movement after periodontal tissue regeneration. Methods Six male beagle dogs were grouped as 1week group,2week group and 4week group randomly. The first premolars were extracted and periodontal defects were made on the mesial aspect of the second premolar. The BMSCs from the dogs were cultured and seeded onto the modified BCP. In experimental group,the BMSCsmodified BCP complex was implanted into periodontal defects in the right upper and left lower quadrant. After 20 weeks of recovery,orthodontic compressional stress was initiated with 1.47 N force. Selfcontrolled experiment was applied,and the other two quadrants were set as 0week group without orthodontic force and control group without neither surgery nor orthodontic force,alternatively. Then two dogs were sacrificed after 1,2 and 4 weeks,and the expressions of NFATc1 and CathK were detected by RealTime PCR and WesternBlot. Results In experimental group,the expression of NFATc1 and CathK in periodontal tissues increased from 0 week to 2 weeks and decreased till 4 weeks. There were no significant differences in expression of these two osteoclastassociate transcript factors between two groups. Conclusions The expression of osteoclastic factors in regenerated periodontal tissue made no difference from that in the healthy periodontal tissue,which brings about the possibility of orthodontic tooth movement after periodontal tissue regeneration.

Key words: biaphasic calcium phosphate, periodontal tissue regeneration, osteoclast, NFATC1, CathK, beagle dogs

中图分类号:

R783.5

吴丽萍宗文逸时函. 再生的牙周组织在正畸压应力下破骨相关因子表达的研究[J]. 口腔医学, 2014, 34(9): 641-645.

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