小肠黏膜下层对牙周膜干细胞体外增殖、骨向分化的影响

›› 2014, Vol. 34 ›› Issue (9): 665-668.

小肠黏膜下层对牙周膜干细胞体外增殖、骨向分化的影响

张红梅¹,荀文兴²,王江³,张晓梅⁴,李蓉²

1. 第四军医大学
2. 第四军医大学唐都医院口腔科
3. 第四军医大学西京医院神经外科
4. 解放军总医院消化内科

收稿日期:2013-05-20 修回日期:2013-07-08 出版日期:2014-09-26 发布日期:2014-10-07
通讯作者: 张红梅 E-mail:zhanghongmei0911@126.com

Effects of small intestinal submucosa on proliferation and osteogenic differentiation of human periodontal ligament stem cells in vitr

Received:2013-05-20 Revised:2013-07-08 Online:2014-09-26 Published:2014-10-07
Contact: Hong-Mei ZHANG E-mail:zhanghongmei0911@126.com

摘要/Abstract

摘要： 目的观察小肠黏膜下层 (SIS)与人牙周膜干细胞(PDLSCs)的生物相容性及其作为支架材料对PDLSCs的骨向分化诱导作用。方法体外分离培养 PDLSCs,分别用四唑盐比色法（MTS）、茜素红染色法、碱性磷酸酶(ALP)和逆转录聚合酶链式反应（RT-PCR）方法观察 SIS 生物材料对PDLSCs增殖活性和骨向分化能力的影响。结果 SIS 显著地刺激体外培养的PDLSCs增殖，诱导了细胞的矿化和提高细胞ALP活性。RT-PCR结果显示SIS诱导后细胞 mRNA 水平表达骨涎蛋白(BSP)和骨钙素（OCN)。结论体外培养条件下，SIS与PDLSCs有良好的生物相容性，能够诱导PDLSCs骨向分化。

关键词: 牙周膜干细胞, 小肠粘膜下层, 增殖, 分化

Abstract: Objective To observe the adhesion and growth of human periodontal ligament stem cells (PDLSCs) seeded on small intestinal submucosa (SIS) in vitro and the osteogenic induction of SIS. Methods PDLSCs were successfully isolated and cultured. The compatibility of the biomaterial was evaluated by MTS assay. The osteogenic differentiation of PDLSCs induced by SIS was measured by using Alizarin red staining,the alkaline phosphatase (ALP) activity and reverse transcriptionpolymerase chain reaction (RTPCR) assay. Results The proliferation of PDLSCs in SIS groups was obviously higher than that in the control groups. The higher levels of ALP activity,the larger mineralizing nods were also detected in the experiment groups. RTPCR demonstrated mRNA expression of bone sialoprotein (BSP) and osteocalcin (OCN) in PDLSCs induced by SIS. Conclusions SIS possesses a good cellular compatibility with PDLSCs. It can induce the osteogenic differentiation of PDLSCs.

Key words: periodontal ligament stem cells, small intestinal submucosa, proliferation, osteogenic differentiation

中图分类号:

R780.2

张红梅荀文兴王江张晓梅李蓉. 小肠黏膜下层对牙周膜干细胞体外增殖、骨向分化的影响[J]. 口腔医学, 2014, 34(9): 665-668.

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