葛根素诱导人骨髓间充质干细胞成骨分化的研究

摘要/Abstract

摘要： ［摘要］目的通过体外实验探讨不同浓度的葛根素诱导骨髓间充质干细胞（bone marrow-mesenchymal stem cells,BMSCs）骨组织分化的作用以及与Notch信号通路之间的关系。方法根据葛根素的浓度，实验分为6组。取3周的SD大鼠分离培养BMSCs，传至第3代后接种细胞，改用不同分组的培养基。继续培养2~4周，测定碱性磷酸酶（ALP）与骨钙素（OC）的含量，评价葛根素的体外诱导成骨能力。用Elisa试剂盒测定Delta样配体4 （DLL4）的含量，评估葛根素的促成骨作用与Notch信号通路的关系。〖HTH〗结果〖HTSS〗不同浓度组的细胞出现与典型成骨细胞相似的形态特征和生物学特性，ALP、OC和DLL4含量增加，但以1×10-5 mol/L组显著高于对照组，比其他浓度组要高，但是差异无统计学意义。〖HTH〗结论〖HTSS〗葛根素能有效促进BMSCs向成骨方向分化，以10-5 mol/L浓度效果最佳，其作用机制可能与Notch信号通路有关。

关键词: 种植义齿 , 骨分化

Abstract: Abstract: Objective To investigate the effects of Puerarin with different concentrations on inducing osteogenic differentiation of bone marrowmesenchymal stem cells （BMSCs）,and to explore the relationship between the mechanism of Puerarins osteoinductivity and the Notch signaling pathway in vitro.Methods There divided 6 groups according to the concentration of Puerarin.After segregated and cultivated for 3 weeks,marrow stromal cells of SD rats in induction group were induced by osteogenesis medium containing different concentrations of Puerarin.In noninduction group,BMSCs were still cultured with DMEM medium.After further cultivation for 2-4 weeks,alkaline phosphatase （ALP）and osteocalcin （OC）were measured to evaluate the in vitro osteoinductivity of Puerarin; Delta like ligand 4 （DLL4）was measured by Elisa kit to determine the relationship between the osteoinductivity and Notch signaling pathway.Results Cells of different concentrations showed morphological and biological characteristics similar to those of osteoblasts.The content of ALP,OC,and DLL4 increased.The increase in the 10-5 mol/L group was more than that of the control group with significant difference and that of other groups of different concentrations with no statistical significance.Conclusions Puerarin can induce BMSCs to differentiate into osteoblasts,and the effect is the best with the concentration of 10-5 mol/L.Meanwhile,its mechanism may be related with Notch signaling pathway.

Key words: implant denture , osteogenic induction

中图分类号:

R329.29

张志明罗善峰. 葛根素诱导人骨髓间充质干细胞成骨分化的研究[J]. , 2015, 35(11): 911-914.

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