AMD3100对口腔鳞状细胞癌细胞生物学行为的影响

›› 2015, Vol. 35 ›› Issue (2): 93-95.

AMD3100对口腔鳞状细胞癌细胞生物学行为的影响

尹东¹,张娜²,袁小超²,韩志刚²,白晓萍²,白晶²

1. 宁夏回族自治区人民医院
2. 宁夏回族自治区人民医院口腔科

收稿日期:2014-07-14 修回日期:2014-10-01 出版日期:2015-02-28 发布日期:2015-03-03
通讯作者: 尹东 E-mail:yindongnx@163.com
基金资助:
宁夏自然科学基金资助项目

Effects of inhibition of CXCR4 antagonist AMD3100 on biological behavior of oral squamous cell carcinoma

Received:2014-07-14 Revised:2014-10-01 Online:2015-02-28 Published:2015-03-03

摘要/Abstract

摘要： 　目的　探讨CXCR4拮抗剂AMD3100对口腔鳞状细胞癌细胞生物学行为的影响。方法采用MTT法检测细胞的增殖能力。通过趋化实验观察CXCR4特异性拮抗剂AMD3100对口腔鳞状细胞癌细胞的侵袭迁移作用。结果 1 ) 口腔鳞状细胞癌细胞在SDF-1作用下增殖反应明显增强，AMD3100可有效抑制肿瘤细胞的增殖。 2 ) 趋化实验显示SDF-1可诱导口腔鳞状细胞癌细胞发生明显迁移， AMD3100能阻断CXCR4受体，从而抑制这种趋化和侵袭转移作用。结论 AMD3100能阻断CXCR 4/SDF-1的相互作用，其可能成为治疗口腔鳞状细胞癌的新方法。

关键词: CXC类趋化因子受体4, 口腔鳞状细胞癌, AMD3100, 增殖, 迁移

Abstract: 　Objective　 To investigate the effect of inhibition of CXCR4 antagonist AMD3100 on biological behavior of oral squamous cell carcinoma(OSCC) cells. Method MTT assay was used to evaluate AMD3100 influence on proliferation of tumor cells. Chemotaxis and migration response to inhibition of the specific CXCR4 antagonist AMD3100 was detected by chemotaxis assay. Results 1) In MTT assay, recombinant SDF-1 stimulated proliferation of tumor cells, and the availability of AMD3100 inhibited proliferation. 2) The chemotactic migration of OSCC cells could be induced by SDF-1. AMD3100 can inhibit the chemotaxis and invasion metastasis by blocking CXCR4 receptors. Conclusion The interaction of CXCR 4/SDF-1 system could be suppressed by AMD3100, and it can be a meaningful therapeutic target and strategy.

Key words: chemokine CXC motif receptor4, oral squamous cell carcinoma, AMD3100, proliferation, migration.

中图分类号:

R739.8，Q279

尹东张娜袁小超韩志刚白晓萍白晶. AMD3100对口腔鳞状细胞癌细胞生物学行为的影响[J]. 口腔医学, 2015, 35(2): 93-95.

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