电纺聚己内酯/Ⅰ型胶原蛋白/纳米羟基磷灰石

摘要/Abstract

摘要： 目的〓〖HT5”SS〗制备聚己内酯/Ⅰ型胶原蛋白/纳米羟基磷灰石复合材料用于可吸收性引导组织再生膜，并进行理化性能表征和生物相容性检测。方法采用静电纺丝技术制备聚己内酯/Ⅰ型胶原蛋白、聚己内酯/Ⅰ型胶原蛋白/纳米羟基磷灰石复合材料，通过扫描电子显微镜表征纤维形貌。牙周膜细胞接种于支架，扫描电镜观察细胞形貌，CCK-8法检测细胞增殖，实时荧光定量PCR检测牙周膜细胞的骨化分化情况。结果聚己内酯/Ⅰ型胶原蛋白、聚己内酯/Ⅰ型胶原蛋白/纳米羟基磷灰石呈复层多孔网状结构，聚己内酯/Ⅰ型胶原蛋白/纳米羟基磷灰石由于纤维内部包裹纳米颗粒致表面呈粗糙外观。扫描电子显微镜、CCK-8法检测技术显示牙周膜细胞在支架上伸展充分，稳定增殖；实时荧光定量PCR结果表明聚己内酯/Ⅰ型胶原蛋白/纳米羟基磷灰石复合材料表面牙周膜细胞中Runt相关转录因子2、骨钙蛋白的基因表达量均明显高于对照组，提示该材料能够诱导牙周膜细胞向成骨细胞方向分化。结论聚己内酯/Ⅰ型胶原蛋白/纳米羟基磷灰石复合材料无明显细胞毒性且具一定的骨化诱导潜能，有望作为一种新型可吸收性引导组织再生膜材料。

关键词: 静电纺丝, 纳米羟基磷灰石, 引导组织再生

Abstract: Objective To prepare the electrospun poly (εcaprolactone)/ typeI collagen/ nanophase hydroxyapatite nanofiber,and to examine its characteristics and biocompatibility.The composite material is expected to be used as the biodegradable membrane for guided tissue regeneration.Methods The PCL/COLⅠ and PCL/COLⅠ/nHAP scaffolds were prepared by electrospinning technique and observed by scanning electronic microscope.The morphology,proliferation and differentiation of periodontal ligament cells seeded on the scaffolds were evaluated by scanning electronic microscope,CCK8 assay and realtime quantitative polymerase chain reaction analysis.Results The PCL/COLⅠ and PCL/COLⅠ/nHAP scaffolds had a porous and interwoven structure,while only the PCL/COLⅠ/nHAP showed a rough surface because of the nHAP particles packaged inside the fibers.The results of the scanning electronic microscope and CCK8 assay showed that periodontal ligament cells adhering to the scaffolds extended adequately and proliferated stably.Additionally,the realtime quantitative polymerase chain reaction analysis revealed that runtrelated transcription factor 2 and osteocalcin of the cells cultured on the PCL/COLⅠ/nHAP expressed higher than that of the control group,which suggested that the PCL/COLⅠ/nHAP scaffold had the ability to induce osteogenic differentiation of periodontal ligament cells.Conclusions The PCL/COLⅠ/nHAP scaffold which has good biocompatibility and osteoinductive ability may serve as a novel biodegradable membrane for guided tissue regeneration.

Key words: electrospinning, nanophase hydroxyapatite, guided tissue regeneration

中图分类号:

R780.2

吴晓楠苗雷英刘玉吴文蕾孙卫斌. 电纺聚己内酯/Ⅰ型胶原蛋白/纳米羟基磷灰石[J]. 口腔医学, 2015, 35(4): 245-249.

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