牙本质屏障细胞毒性试验的研究进展

›› 2015, Vol. 35 ›› Issue (4): 302-305.

牙本质屏障细胞毒性试验的研究进展

蒋若丹¹,林红²

1. 北京大学口腔医学院口腔医疗器械检验中心口腔材料研究室口腔数字化医疗技术和材料国家工程实验室
2. 北京大学口腔医学院口腔材料研究室

收稿日期:2014-11-27 修回日期:2014-12-26 出版日期:2015-04-28 发布日期:2015-04-27
通讯作者: 蒋若丹 E-mail:daisyjrd@163.com
基金资助:
国家科技支撑计划

The research progress of dentin barrier cytotoxicity test

Received:2014-11-27 Revised:2014-12-26 Online:2015-04-28 Published:2015-04-27
Contact: Ruo-Dan JIANG E-mail:daisyjrd@163.com

摘要/Abstract

摘要： 牙本质屏障细胞毒性试验能较好地模拟临床实际，在评价牙体充填材料及与牙本质直接接触材料细胞毒性方面有很大的价值，具有替代动物试验的可能性，并已广泛应用于相关材料的毒性研究。该文就此试验中细胞的选择与培养、试验装置和牙本质片的选取等主要关键技术点做一综述。

关键词: 细胞毒性, 牙本质屏障试验, 牙本质通透性, 三维细胞培养

Abstract: Dentin barrier cytotoxicity test which can mimic the clinical situation plays an important role in evaluating the toxicity of dentin filling materials and dentin contacting materials.It has been widely used in the toxicity research of related materials and has the possibility of replacing the animal test.This article reviewed the key technique points involving the choice and culture of cells,the test device and the selection of dentin disks.

Key words: cytotoxicity, dentine barrier test, dentin permeability, threedimensional cell culture

中图分类号:

R394.26

蒋若丹林红. 牙本质屏障细胞毒性试验的研究进展[J]. 口腔医学, 2015, 35(4): 302-305.

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